PDF(Pubmed)
Abstract：
In recent times, the pathogenesis of generalized anxiety disorder (GAD) and the influence of pro- and anti-inflammatory cytokines on it have garnered considerable interest. Cytokine research, especially Th-17 cytokine research on GAD patients, is limited. Here, we aim to assess the role of interleukin-17A (IL-17A) and interleukin-23A (IL-23A) in the pathophysiology and development of GAD. This investigation included 50 GAD patients and 38 age-sex-matched healthy controls (HCs). A psychiatrist diagnosed patients with GAD and assessed symptom severity using the DSM-5 and the GAD-7 scales. The serum concentrations of IL-17A and IL-23A were determined using commercially available ELISA kits. GAD patients exhibited elevated levels of IL-17A (77.14 ± 58.30 pg/ml) and IL-23A (644.90 ± 296.70 pg/ml) compared to HCs (43.50 ± 25.54 pg/ml and 334.40 ± 176.0 pg/ml). We observed a positive correlation between disease severity and cytokine changes (IL-23A: r = 0.359, p = 0.039; IL-17A: r = 0.397, p = 0.032). These findings indicate that IL-17A and IL-23A may be associated with the pathophysiology of GAD. ROC analysis revealed moderately higher AUC values (IL-23A: 0.824 and IL-17A: 0.710), demonstrating their potential to discriminate between patients and HCs. Also, the sensitivity values of both cytokines were relatively higher (IL-23A: 80.49% and IL-17A: 77.27%). According to the present findings, there may be an association between peripheral serum levels of IL-17A and IL-23A and the pathophysiology and development of GAD. These altered serum IL-17A and IL-23A levels may play a role in directing the early risk of developing GAD. We recommend further research to ascertain their exact role in the pathophysiology and their performance as risk assessment markers of GAD.
摘要：
最近,广泛性焦虑症（GAD）的发病机制以及促炎和抗炎细胞因子对其的影响引起了人们的极大兴趣。细胞因子研究,特别是Th-17细胞因子对GAD患者的研究，是有限的。这里,我们旨在评估白细胞介素-17A(IL-17A)和白细胞介素-23A(IL-23A)在GAD病理生理和发展中的作用.这项调查包括50名GAD患者和38名年龄性别匹配的健康对照（HCs）。精神科医生诊断为GAD患者，并使用DSM-5和GAD-7量表评估症状严重程度。使用市售ELISA试剂盒测定IL-17A和IL-23A的血清浓度。GAD患者与HC（43.50±25.54pg/ml和334.40±176.0pg/ml）相比，IL-17A（77.14±58.30pg/ml）和IL-23A（644.90±296.70pg/ml）的水平升高。我们观察到疾病严重程度与细胞因子变化之间存在正相关（IL-23A：r=0.359，p=0.039；IL-17A：r=0.397，p=0.032）。这些发现表明IL-17A和IL-23A可能与GAD的病理生理有关。ROC分析显示AUC值稍高（IL-23A：0.824和IL-17A：0.710），展示了他们区分患者和HCs的潜力。此外，两种细胞因子的敏感性值相对较高（IL-23A：80.49％和IL-17A：77.27％）。根据目前的发现,外周血IL-17A和IL-23A水平可能与GAD的病理生理和发展有关。这些改变的血清IL-17A和IL-23A水平可能在指导发展GAD的早期风险中起作用。我们建议进一步研究，以确定它们在病理生理学中的确切作用及其作为GAD风险评估标志物的性能。
