Abstract：
Northeastern Algeria boasts numerous hot springs, yet these hydrothermal sites remain largely unexplored for their microbial ecology. The present study explores the bacterial abundance and diversity within two distinct Algerian hot springs (Hammam Saïda and Hammam Debagh) and investigates the link between the prevailing bacteria with geochemical parameters. High-throughput 16S rRNA gene sequencing of water and sediment samples revealed a bacterial dominance of 99.85-91.16% compared to Archaea (0.14-0.66%) in both springs. Interestingly, Saïda hot spring, characterized by higher temperatures and sodium content, harbored a community dominated by Pseudomonadota (51.13%), whereas Debagh, a Ca-Cl-SO4 type spring, was primarily populated by Bacillota with 55.33%. Bacteroidota displayed even distribution across both sites. Additional phyla, including Chloroflexota, Deinococcota, Cyanobacteriota, and Chlorobiota, were also present. Environmental factors, particularly temperature, sodium, potassium, and alkalinity, significantly influenced bacterial diversity and composition. These findings shed light on the interplay between distinct microbial communities and their associated geochemical properties, providing valuable insights for future research on biogeochemical processes in these unique ecosystems driven by distinct environmental conditions, including potential applications in bioremediation and enzyme discovery.
摘要：
阿尔及利亚东北部拥有众多温泉，然而，这些热液场所在很大程度上仍未探索其微生物生态。本研究探讨了两个不同的阿尔及利亚温泉（HammamamSaida和HammamDebagh）中的细菌丰度和多样性，并研究了盛行细菌与地球化学参数之间的联系。水和沉积物样品的高通量16SrRNA基因测序显示，在两个春季，细菌的优势均为99.85-91.16％，而古菌（0.14-0.66％）。有趣的是,赛达温泉，具有较高的温度和钠含量，藏有一个由Pseudomonadota（51.13％）主导的社区，而Debagh,Ca-Cl-SO4型弹簧，主要由芽孢杆菌居住，占55.33%。拟杆菌在两个站点上的分布均匀。额外的门，包括氯氟科,Deinococcota,蓝细菌,和叶绿素,也在场。环境因素,特别是温度,钠,钾，和碱度，显著影响细菌多样性和组成。这些发现揭示了不同微生物群落及其相关地球化学性质之间的相互作用，为未来在不同环境条件驱动的这些独特生态系统中的生物地球化学过程的研究提供有价值的见解，包括在生物修复和酶发现中的潜在应用。
