Abstract：
OBJECTIVE: Several treatment options for acne vulgaris are limited by their associated adverse effects. An innovative approach involves introducing light-absorbing nanoparticles into sebaceous follicles before destroying the follicles using selective photothermolysis. We aimed to investigate efficient methods for introducing gold and platinum nanoparticles into sebaceous follicles and to identify suitable laser equipment and parameters for the effective destruction of these follicles.
METHODS: We used porcine skin as the experimental model. We compared the efficacies of a thulium laser, ultrasound, and manual massage and evaluated the optimal method for delivering nanoparticles in close proximity to sebaceous follicles. Subsequently, a 1064-nm-wavelength neodymium-doped yttrium aluminum garnet (Nd: YAG) laser was employed to induce selective photothermolysis. We compared different parameters to identify the optimal pulse duration and fluence of the Nd: YAG laser. The extent of penetration and destruction of sebaceous follicles was assessed using hematoxylin and eosin (H&E) staining, and a numerical evaluation was conducted.
RESULTS: H&E staining showed that irradiation with a long-pulsed Nd: YAG laser following a combination of thulium laser and sonophoresis effectively destroyed sebaceous follicles, with destruction rates exceeding 50%. These results were valid with a long pulse duration and a high fluence of the Nd: YAG laser.
CONCLUSIONS: This study demonstrated that sebaceous follicles can be effectively destroyed through a mixture of gold and platinum nanoparticle delivery by a combination of microchanneling and sonophoresis, followed by selective thermal damage induced by a 1064-nm long-pulsed high-fluence Nd: YAG laser.
METHODS: We used porcine skin as the experimental model. We compared the efficacies of a thulium laser, ultrasound, and manual massage and evaluated the optimal method for delivering nanoparticles in close proximity to sebaceous follicles. Subsequently, a 1064-nm-wavelength neodymium-doped yttrium aluminum garnet (Nd: YAG) laser was employed to induce selective photothermolysis. We compared different parameters to identify the optimal pulse duration and fluence of the Nd: YAG laser. The extent of penetration and destruction of sebaceous follicles was assessed using hematoxylin and eosin (H&E) staining, and a numerical evaluation was conducted.
RESULTS: H&E staining showed that irradiation with a long-pulsed Nd: YAG laser following a combination of thulium laser and sonophoresis effectively destroyed sebaceous follicles, with destruction rates exceeding 50%. These results were valid with a long pulse duration and a high fluence of the Nd: YAG laser.
CONCLUSIONS: This study demonstrated that sebaceous follicles can be effectively destroyed through a mixture of gold and platinum nanoparticle delivery by a combination of microchanneling and sonophoresis, followed by selective thermal damage induced by a 1064-nm long-pulsed high-fluence Nd: YAG laser.
摘要：
目的：寻常痤疮的几种治疗方案因其相关不良反应而受到限制。一种创新的方法涉及在使用选择性光热解破坏毛囊之前将光吸收纳米颗粒引入皮脂腺毛囊中。我们旨在研究将金和铂纳米颗粒引入皮脂腺毛囊的有效方法，并确定有效破坏这些毛囊的合适激光设备和参数。
方法:我们使用猪皮肤作为实验模型。我们比较了thu激光的功效，超声，和手动按摩，并评估了在皮脂腺毛囊附近递送纳米颗粒的最佳方法。随后，使用1064nm波长的掺钕钇铝石榴石（Nd：YAG）激光诱导选择性光热解。我们比较了不同的参数，以确定Nd：YAG激光的最佳脉冲持续时间和通量。使用苏木精和伊红（H&E）染色评估皮脂腺毛囊的渗透和破坏程度，并进行了数值评估。
结果：H&E染色显示，长脉冲Nd:YAG激光照射后，使用thu激光和超声电泳的组合有效地破坏了皮脂腺毛囊，破坏率超过50%。这些结果对于Nd：YAG激光的长脉冲持续时间和高通量是有效的。
结论：这项研究表明，通过微通道和超声电泳的组合，可以通过金和铂纳米颗粒的混合物有效地破坏皮脂腺毛囊，随后是1064nm长脉冲高通量Nd：YAG激光引起的选择性热损伤。
方法:我们使用猪皮肤作为实验模型。我们比较了thu激光的功效，超声，和手动按摩，并评估了在皮脂腺毛囊附近递送纳米颗粒的最佳方法。随后，使用1064nm波长的掺钕钇铝石榴石（Nd：YAG）激光诱导选择性光热解。我们比较了不同的参数，以确定Nd：YAG激光的最佳脉冲持续时间和通量。使用苏木精和伊红（H&E）染色评估皮脂腺毛囊的渗透和破坏程度，并进行了数值评估。
结果：H&E染色显示，长脉冲Nd:YAG激光照射后，使用thu激光和超声电泳的组合有效地破坏了皮脂腺毛囊，破坏率超过50%。这些结果对于Nd：YAG激光的长脉冲持续时间和高通量是有效的。
结论：这项研究表明，通过微通道和超声电泳的组合，可以通过金和铂纳米颗粒的混合物有效地破坏皮脂腺毛囊，随后是1064nm长脉冲高通量Nd：YAG激光引起的选择性热损伤。
