Abstract：
A Gram-negative, rod-shaped, non-motile, aerobic bacterium, designated as strain TK19101T, was isolated from the intermediate seawater of yellow vent in the shallow-sea hydrothermal system located near Kueishantao Island. The strain was found to grow at 10-40 °C (optimum, 35 °C), at pH 6.0-8.0 (optimum, 7.0), and in 0-5% (w/v) NaCl (optimum, 1%). Strain TK19101T was catalase-positive and oxidase-positive. The predominant fatty acids (> 10%) in strain TK19101T cells were C16:0, summed feature 8 (C18:1 ω6c and/or C18:1 ω7c), and C18:0. The predominant isoprenoid quinone of strain TK19101T was ubiquinone-10. The polar lipids of strain TK19101T comprised phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phospholipid, and unknown polar lipid. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TK19101T belonged to the genus Mesobacterium. Strain TK19101T exhibited highest 16S rRNA gene sequence similarity value to Mesobacterium pallidum MCCC M24557T (97.48%). The estimated average nucleotide identity and digital DNA-DNA hybridization values between strain TK19101T and the closest related species Mesobacterium pallidum MCCC M24557T were 74.88% and 20.30%, respectively. The DNA G + C content was 63.49 mol%. On the basis of the analysis of 16S rRNA gene sequences, genotypic and phylogenetic data, strain TK19101T has a unique phylogenetic status and represents a novel species of genus Mesobacterium, for which the name Mesobacterium hydrothermale sp. nov. is proposed. The type strain is TK19101T (= MCCC 1K08936T = KCTC 8354T).
摘要：
革兰氏阴性，杆状,不活动，需氧细菌，命名为菌株TK19101T，从位于奎山陶岛附近的浅海热液系统中黄色喷口的中间海水中分离出来。发现该菌株在10-40°C下生长（最佳，35°C),在pH6.0-8.0(最佳，7.0),和0-5%(w/v)NaCl(最佳，1%)。菌株TK19101T为过氧化氢酶阳性和氧化酶阳性。菌株TK19101T细胞中的主要脂肪酸（>10％）为C16：0，总计特征8（C18：1ω6c和/或C18：1ω7c），C18:0菌株TK19101T的主要类异戊二烯醌是泛醌-10。菌株TK19101T的极性脂质包括磷脂酰胆碱，磷脂酰乙醇胺，磷脂酰甘油，磷脂，和未知的极性脂质。基于16SrRNA基因序列的系统发育分析表明，菌株TK19101T属于中生杆菌属。菌株TK19101T与梅毒MCCCM24557T表现出最高的16SrRNA基因序列相似性值(97.48%)。菌株TK19101T和最接近的近缘种MCCCM24557T之间的估计平均核苷酸同一性和数字DNA-DNA杂交值分别为74.88％和20.30％，分别。DNAG+C含量为63.49mol%。在分析16SrRNA基因序列的基础上,基因型和系统发育数据，菌株TK19101T具有独特的系统发育状态，代表了中生杆菌属的新物种，为此，命名为Mesobacteriumhydrothermalesp。11月。是提议的。菌株类型为TK19101T(=MCCC1K08936T=KCTC8354T)。
