Abstract：
CONCLUSIONS: This comprehensive review underscores the application of genome editing in plant reproductive biology, including recent advances and challenges associated with it. Genome editing (GE) is a powerful technology that has the potential to accelerate crop improvement by enabling efficient, precise, and rapid engineering of plant genomes. Over the last decade, this technology has rapidly evolved from the use of meganucleases (homing endonucleases), zinc-finger nucleases, transcription activator-like effector nucleases to the use of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (CRISPR/Cas), which has emerged as a popular GE tool in recent times and has been extensively used in several organisms, including plants. GE has been successfully employed in several crops to improve plant reproductive traits. Improving crop reproductive traits is essential for crop yields and securing the world\'s food supplies. In this review, we discuss the application of GE in various aspects of plant reproductive biology, including its potential application in haploid induction, apomixis, parthenocarpy, development of male sterile lines, and the regulation of self-incompatibility. We also discuss current challenges and future prospects of this technology for crop improvement, focusing on plant reproduction.
摘要：
结论：这篇综合综述强调了基因组编辑在植物生殖生物学中的应用，包括与之相关的最新进展和挑战。基因组编辑（GE）是一项强大的技术，有可能通过实现高效，加速作物改良。精确，和植物基因组的快速工程。在过去的十年里，这项技术已经从使用大范围核酸酶（归巢核酸内切酶）迅速发展起来，锌指核酸酶，转录激活因子样效应核酸酶使用成簇的规则间隔短回文重复序列（CRISPR）/CRISPR相关蛋白（CRISPR/Cas），它在最近一段时间已经成为一种流行的GE工具，并已被广泛用于几种生物，包括植物。GE已成功用于几种作物，以改善植物的繁殖特性。改善作物的繁殖特性对于作物产量和确保世界粮食供应至关重要。在这次审查中,我们讨论了GE在植物生殖生物学各个方面的应用，包括其在单倍体诱导中的潜在应用，无融合生殖，单性结实,雄性不育系的发展，和自我不相容性的调节。我们还讨论了该技术用于作物改良的当前挑战和未来前景，专注于植物繁殖。
