Abstract：
To understand whether domestication had an impact on susceptibility and responsiveness to arbuscular mycorrhizal fungi (AMF) in tomato (Solanum lycopersicum), we investigated two tomato cultivars (\"M82\" and \"Moneymaker\") and a panel of wild relatives including S. neorickii, S. habrochaites and S. pennellii encompassing the whole Lycopersicon clade. Most genotypes revealed good AM colonisation levels when inoculated with the AMF Funneliformis mosseae. By contrast, both S. pennellii accessions analysed showed a very low colonisation, but with normal arbuscule morphology, and a negative response in terms of root and shoot biomass. This behaviour was independent of fungal identity and environmental conditions. Genomic and transcriptomic analyses revealed in S. pennellii the lack of genes identified within QTLs for AM colonisation, a limited transcriptional reprogramming upon mycorrhization and a differential regulation of strigolactones and AM-related genes compared to tomato. Donor plants experiments indicated that the AMF could represent a cost for S. pennellii: F. mosseae could extensively colonise the root only when it was part of a mycorrhizal network, but a higher mycorrhization led to a higher inhibition of plant growth. These results suggest that genetics and functional traits of S. pennellii are responsible for the limited extent of AMF colonisation.
摘要：
为了了解驯化是否对番茄（Solanumlycopersicum）中丛枝菌根真菌（AMF）的敏感性和反应性产生影响，我们调查了两个番茄品种（“M82”和“Moneymaker”）和一组野生近缘种，包括S.neorickii，S.habrochaites和S.pennellii涵盖了整个Lycopersicon进化枝。接种AMF真菌时，大多数基因型都显示出良好的AM定植水平。相比之下,所分析的两个美国pennellii种质都显示出非常低的殖民化，但是具有正常的丛状形态，以及根和芽生物量方面的负响应。这种行为与真菌身份和环境条件无关。基因组和转录组分析揭示了在S.pennellii中缺乏在QTL中鉴定的用于AM定植的基因，与番茄相比，菌根化过程中有限的转录重编程以及对str金内酯和AM相关基因的差异调节。供体植物实验表明，AMF可能代表S.pennellii的成本：F.mosseae只有当它是菌根网络的一部分时才能广泛定植根，但是较高的菌根化导致对植物生长的较高抑制作用。这些结果表明，彭氏链球菌的遗传和功能特征是AMF定殖程度有限的原因。
