PDF(Pubmed)
Abstract：
Exploring the effects of seasonal variation on the gut microbiota of cold-water fish plays an important role in understanding the relationship between seasonal variation and cold-water fish. Gut samples of cold-water fish and environmental samples were collected during summer and winter from the lower reaches of the Yalong River. The results of the 16S rRNA sequencing showed that significant differences were identified in the composition and diversity of gut bacteria of cold-water fish. Co-occurrence network complexity of the gut bacteria of cold-water fish was higher in summer compared to winter (Sum: nodes: 256; edges: 20,450; Win: nodes: 580; edges: 16,725). Furthermore, from summer to winter, the contribution of sediment bacteria (Sum: 5.3%; Win: 23.7%) decreased in the gut bacteria of cold-water fish, while the contribution of water bacteria (Sum: 0%; Win: 27.7%) increased. The normalized stochastic ratio (NST) and infer community assembly mechanisms by phylogenetic bin-based null model analysis (iCAMP) showed that deterministic processes played a more important role than stochastic processes in the microbial assembly mechanism of gut bacteria of cold-water fish. From summer to winter, the contribution of deterministic processes to gut bacteria community assembly mechanisms decreased, while the contribution of stochastic processes increased. Overall, these results demonstrated that seasonal variation influenced the gut bacteria of cold-water fish and served as a potential reference for future research to understand the adaptation of fish to varying environments.
摘要：
探索季节变化对冷水鱼肠道菌群的影响对于理解季节变化与冷水鱼的关系具有重要作用。夏季和冬季从亚龙河下游收集了冷水鱼的肠道样本和环境样本。16SrRNA测序结果表明，冷水鱼肠道细菌的组成和多样性存在显着差异。与冬季相比，夏季冷水鱼的肠道细菌的共现网络复杂性更高（总和：节点：256；边缘：20,450；Win：节点：580；边缘：16,725）。此外，从夏天到冬天,在冷水鱼的肠道细菌中，沉积物细菌的贡献（总和：5.3％；Win：23.7％）减少，而水中细菌的贡献(总和:0%；Win:27.7%)增加。归一化随机比率（NST）和基于系统发育bin的零模型分析（iCAMP）推断群落组装机制表明，确定性过程在冷水鱼肠道细菌的微生物组装机制中起着比随机过程更重要的作用。从夏天到冬天，确定性过程对肠道细菌群落组装机制的贡献下降，而随机过程的贡献增加。总的来说,这些结果表明，季节性变化影响了冷水鱼的肠道细菌，并为未来研究了解鱼类对不同环境的适应性提供了潜在的参考。
