PDF(Pubmed)
Abstract：
UNASSIGNED: Weeds are significant factors that detrimentally affect crop health and hinder optimal herbage yield. Rhizosphere microorganisms play crucial roles in plant growth, development, and nutrient uptake. Therefore, research focusing on weed control through the lens of microorganisms has emerged as a prominent area of study. The oil-producing fungus Mortierella, which is known for its numerous agricultural benefits, has garnered significant attention in recent years.
UNASSIGNED: In this study, we conducted inoculation experiments in a controlled artificial culture climate chamber to investigate the effects of differential hormones and differentially expressed genes in the stems and leaves of Digitaria sanguinalis using Liquid Chromatography Tandem Mass Spectrometry and RNA-seq techniques, respectively. Additionally, Pearson\'s correlation analysis was used to establish correlations between differential hormones and growth indicators of Digitaria sanguinalis.
UNASSIGNED: The results demonstrated that inoculation with Mortierella sp. MXBP304 effectively suppressed aboveground biomass and plant height in Digitaria sanguinalis. Furthermore, there was significant upregulation and downregulation in the expression of genes involved in the synthesis and metabolism of phenylalanine and L-phenylalanine. Conversely, the expression of genes related to tryptophan, L-tryptophan, and indole was significantly downregulated. The addition of Mortierella sp. MXBP304 can influence the gene expression associated with phenylalanine and tryptophan synthesis and metabolism during Digitaria sanguinalis growth, subsequently reducing the relative contents of phenylalanine and tryptophan, thereby directly inhibiting Digitaria sanguinalis growth.
UNASSIGNED: In this study, we conducted inoculation experiments in a controlled artificial culture climate chamber to investigate the effects of differential hormones and differentially expressed genes in the stems and leaves of Digitaria sanguinalis using Liquid Chromatography Tandem Mass Spectrometry and RNA-seq techniques, respectively. Additionally, Pearson\'s correlation analysis was used to establish correlations between differential hormones and growth indicators of Digitaria sanguinalis.
UNASSIGNED: The results demonstrated that inoculation with Mortierella sp. MXBP304 effectively suppressed aboveground biomass and plant height in Digitaria sanguinalis. Furthermore, there was significant upregulation and downregulation in the expression of genes involved in the synthesis and metabolism of phenylalanine and L-phenylalanine. Conversely, the expression of genes related to tryptophan, L-tryptophan, and indole was significantly downregulated. The addition of Mortierella sp. MXBP304 can influence the gene expression associated with phenylalanine and tryptophan synthesis and metabolism during Digitaria sanguinalis growth, subsequently reducing the relative contents of phenylalanine and tryptophan, thereby directly inhibiting Digitaria sanguinalis growth.
摘要：
■杂草是有害地影响作物健康并阻碍最佳牧草产量的重要因素。根际微生物在植物生长中起着至关重要的作用,发展,和营养吸收。因此,通过微生物晶状体进行杂草控制的研究已成为一个突出的研究领域。产油真菌被孢霉，以其众多的农业效益而闻名，近年来引起了极大的关注。
■在这项研究中，我们在受控的人工培养气候室中进行了接种实验，以使用液相色谱-串联质谱法和RNA-seq技术研究血洋地黄茎和叶中差异激素和差异表达基因的影响。分别。此外,采用Pearson相关分析建立不同激素与血洋地黄生长指标的相关性。
■结果表明接种被孢霉。MXBP304有效抑制了血洋地黄的地上生物量和株高。此外，参与苯丙氨酸和L-苯丙氨酸合成和代谢的基因表达存在显著上调和下调。相反，色氨酸相关基因的表达，L-色氨酸,吲哚显著下调。被孢霉的添加。MXBP304可影响血洋地黄生长过程中与苯丙氨酸和色氨酸合成和代谢相关的基因表达，随后降低苯丙氨酸和色氨酸的相对含量，从而直接抑制血洋地黄的生长。
■在这项研究中，我们在受控的人工培养气候室中进行了接种实验，以使用液相色谱-串联质谱法和RNA-seq技术研究血洋地黄茎和叶中差异激素和差异表达基因的影响。分别。此外,采用Pearson相关分析建立不同激素与血洋地黄生长指标的相关性。
■结果表明接种被孢霉。MXBP304有效抑制了血洋地黄的地上生物量和株高。此外，参与苯丙氨酸和L-苯丙氨酸合成和代谢的基因表达存在显著上调和下调。相反，色氨酸相关基因的表达，L-色氨酸,吲哚显著下调。被孢霉的添加。MXBP304可影响血洋地黄生长过程中与苯丙氨酸和色氨酸合成和代谢相关的基因表达，随后降低苯丙氨酸和色氨酸的相对含量，从而直接抑制血洋地黄的生长。
