Abstract：
The occurrence of cancer is often closely related to multiple tumor markers, so it is important to develop multitarget detection methods. By the proper design of the input signals and logical operations of DNA logic gates, detection and diagnosis of cancer at different stages can be achieved. For example, in the early stages, specific input signals can be designed to correspond to early specific tumor markers, thereby achieving early cancer detection. In the late stage, logic gates for multitarget detection can be designed to simultaneously detect multiple biomarkers to improve diagnostic accuracy and comprehensiveness. In this work, we constructed a dual-target-triggered DNA logic gate for anchoring DNA tetrahedra, where methylene blue was embedded in the DNA tetrahedra to sensitize ZnO@CdS@Au, achieving ultrasensitive detection of the target substance. We tested the response of AND and OR logic gates to the platform. For AND logic gates, the sensing platform only responds when both miRNAs are present. In the concentration range of 10 aM to 10 nM, the photoelectric signal gradually increases with an increase of the target concentration. Subsequently, we used OR logic gates for miRNA detection. Even if only one target exists, the sensing platform exhibits excellent performance. Similarly, within the concentration range of 10 aM to 10 nM, the photoelectric signal gradually increases with an increase of the target concentration. The minimum detection limit is 1.10 aM. Whether it is the need to detect multiple targets simultaneously or only one of them, we can achieve it by selecting the appropriate logic gate. This strategy holds promising application prospects in fields such as biosensing, medical diagnosis, and environmental monitoring.
摘要：
肿瘤的发生往往与多种肿瘤标志物密切相关，因此发展多目标检测方法具有重要意义。通过对DNA逻辑门的输入信号和逻辑运算的适当设计，可以实现不同阶段癌症的检测和诊断。例如,在早期阶段，特定的输入信号可以设计为对应于早期特定的肿瘤标志物，从而实现癌症的早期检测。在后期阶段,用于多目标检测的逻辑门可以设计为同时检测多个生物标志物，以提高诊断的准确性和全面性。在这项工作中,我们构建了一个双目标触发的DNA逻辑门，用于锚定DNA四面体，其中亚甲基蓝嵌入DNA四面体中以敏化ZnO@CdS@Au，实现对目标物质的超灵敏检测。我们测试了AND和OR逻辑门对平台的响应。对于AND逻辑门，传感平台仅在两种miRNA都存在时才响应。在10aM至10nM的浓度范围内，光电信号随着靶浓度的增加而逐渐增加。随后，我们使用OR逻辑门进行miRNA检测。即使只有一个目标存在,传感平台表现出优异的性能。同样，在10aM至10nM的浓度范围内，光电信号随着靶浓度的增加而逐渐增加。最低检测限为1.10aM。无论是需要同时检测多个目标还是只检测其中一个目标,我们可以通过选择合适的逻辑门来实现它。该策略在生物传感等领域具有广阔的应用前景。医学诊断,和环境监测。
