PDF(Pubmed)
Abstract：
BACKGROUND: Physical activity is associated with improved disease progression and cancer-specific survival in patients with prostate cancer (PCa). However, the mechanisms underlying these associations remain unclear, while the relative impact of exercise modes is unknown. This study aims to examine the differential impact of exercise mode on tumour-suppressive skeletal muscle-associated systemic molecules as well as their delivery mechanism. This study will compare the effects of the two main exercise modes, aerobic and resistance, on (1) circulatory myokine levels, (2) skeletal muscle-induced extracellular vesicle abundance and cargo contents, and (3) uptake of extracellular vesicles (EVs) in PCa cells in patients with localised or advanced PCa.
METHODS: A single-group cross-over design will be used for patients at opposite ends of the disease spectrum. A total of 32 patients (localised PCa, n = 16; metastatic castrate-resistant PCa, n = 16) will be recruited while capitalising on two ongoing studies. Ethics amendment has been approved for two ongoing trials to share data, implement the acute exercise sessions, and collect additional blood samples from patients. The patients will undertake two exercise sessions (aerobic only and resistance only) in random order one week apart. Blood will be collected before, after, and 30 min post-exercise. Circulating/EV-contained myokine levels (irisin, IL-6, IL-15, FGF-21, and SPARC) and plasma skeletal muscle-induced EVs will be measured using ELISA and flow cytometry. PCa cell line growth with or without collected plasma will be examined using PCa cell lines (LNCaP, DU-145, and PC-3), while evaluating cellular uptake of EVs. Ethics amendments have been approved for two capitalising studies to share data, implement acute exercise sessions and collect additional samples from the patients.
CONCLUSIONS: If findings show a differential impact of exercise mode on the establishment of an anti-cancer systemic environment, this will provide fundamental knowledge for developing targeted exercise prescriptions for patients with PCa across different disease stages. Findings will be reported in peer-reviewed publications and scientific conferences, in addition to working with national support groups to translate findings for the broader community.
BACKGROUND: The registration for the two capitalising studies are NCT02730338 and ACTRN12618000225213.
METHODS: A single-group cross-over design will be used for patients at opposite ends of the disease spectrum. A total of 32 patients (localised PCa, n = 16; metastatic castrate-resistant PCa, n = 16) will be recruited while capitalising on two ongoing studies. Ethics amendment has been approved for two ongoing trials to share data, implement the acute exercise sessions, and collect additional blood samples from patients. The patients will undertake two exercise sessions (aerobic only and resistance only) in random order one week apart. Blood will be collected before, after, and 30 min post-exercise. Circulating/EV-contained myokine levels (irisin, IL-6, IL-15, FGF-21, and SPARC) and plasma skeletal muscle-induced EVs will be measured using ELISA and flow cytometry. PCa cell line growth with or without collected plasma will be examined using PCa cell lines (LNCaP, DU-145, and PC-3), while evaluating cellular uptake of EVs. Ethics amendments have been approved for two capitalising studies to share data, implement acute exercise sessions and collect additional samples from the patients.
CONCLUSIONS: If findings show a differential impact of exercise mode on the establishment of an anti-cancer systemic environment, this will provide fundamental knowledge for developing targeted exercise prescriptions for patients with PCa across different disease stages. Findings will be reported in peer-reviewed publications and scientific conferences, in addition to working with national support groups to translate findings for the broader community.
BACKGROUND: The registration for the two capitalising studies are NCT02730338 and ACTRN12618000225213.
摘要：
背景：体力活动与前列腺癌（PCa）患者疾病进展和癌症特异性生存期的改善相关。然而,这些关联背后的机制仍不清楚，而运动模式的相对影响是未知的。这项研究旨在研究运动模式对肿瘤抑制性骨骼肌相关全身分子及其传递机制的不同影响。本研究将比较两种主要运动模式的效果，有氧和抗性，关于（1）循环肌力水平，(2)骨骼肌诱导的细胞外囊泡丰度和货物含量,和(3)局部或晚期PCa患者PCa细胞中细胞外囊泡(EV)的摄取。
方法：将对疾病谱两端的患者采用单组交叉设计。共有32名患者(局部PCa，n=16；转移性去势抗性PCa，n=16）将被招募，同时利用两项正在进行的研究。伦理修正案已被批准用于两项正在进行的试验，以共享数据，实施急性锻炼，并从病人身上收集额外的血样。患者将每隔一周随机进行两次运动（仅有氧运动和仅抵抗运动）。之前会收集血液，之后,运动后30分钟。循环/EV包含的肌动蛋白水平(irisin，IL-6，IL-15，FGF-21和SPARC）和血浆骨骼肌诱导的EV将使用ELISA和流式细胞术进行测量。使用PCa细胞系（LNCaP，DU-145和PC-3)，同时评估电动汽车的细胞摄取。伦理修正案已被批准用于两项资本化研究，以共享数据，实施急性锻炼，并从患者中收集额外的样本。
结论：如果研究结果表明运动模式对建立抗癌系统环境的不同影响，这将为针对不同疾病阶段的PCa患者制定有针对性的运动处方提供基础知识.研究结果将在同行评审的出版物和科学会议上报告，除了与国家支持团体合作，为更广泛的社区翻译调查结果。
背景：两项大写研究的注册是NCT02730338和ACTRN12618000225213。
方法：将对疾病谱两端的患者采用单组交叉设计。共有32名患者(局部PCa，n=16；转移性去势抗性PCa，n=16）将被招募，同时利用两项正在进行的研究。伦理修正案已被批准用于两项正在进行的试验，以共享数据，实施急性锻炼，并从病人身上收集额外的血样。患者将每隔一周随机进行两次运动（仅有氧运动和仅抵抗运动）。之前会收集血液，之后,运动后30分钟。循环/EV包含的肌动蛋白水平(irisin，IL-6，IL-15，FGF-21和SPARC）和血浆骨骼肌诱导的EV将使用ELISA和流式细胞术进行测量。使用PCa细胞系（LNCaP，DU-145和PC-3)，同时评估电动汽车的细胞摄取。伦理修正案已被批准用于两项资本化研究，以共享数据，实施急性锻炼，并从患者中收集额外的样本。
结论：如果研究结果表明运动模式对建立抗癌系统环境的不同影响，这将为针对不同疾病阶段的PCa患者制定有针对性的运动处方提供基础知识.研究结果将在同行评审的出版物和科学会议上报告，除了与国家支持团体合作，为更广泛的社区翻译调查结果。
背景：两项大写研究的注册是NCT02730338和ACTRN12618000225213。
