Abstract：
This chapter outlines the workflow using the ExpiSf™ Expression System designed for high-density infection of suspension ExpiSf9™ cells. The system utilizes a chemically defined, serum-free, protein-free, and animal origin free medium, making it suitable for recombinant protein expression experiments. The ExpiSf™ chemically defined medium allows efficient transfection and baculovirus production directly within the same culture medium. The ExpiSf™ Expression System Starter Kit provides all necessary components, including cells, culture medium, and reagents needed to infect one (1) liter of cell culture. The system\'s versatility and animal origin free nature make it a valuable tool for various protein expression studies and biotechnological applications.
摘要：
本章概述了使用ExpiSf™表达系统的工作流程，该系统设计用于高密度感染悬浮ExpiSf9™细胞。该系统利用化学定义，无血清,无蛋白质,和无动物来源的培养基，使其适用于重组蛋白表达实验。ExpiSf™化学成分确定的培养基允许在同一培养基中直接进行有效转染和杆状病毒生产。ExpiSf™Expression系统入门套件提供所有必要的组件，包括细胞,培养基,以及感染一（1）升细胞培养物所需的试剂。该系统的多功能性和动物来源自由的性质使其成为各种蛋白质表达研究和生物技术应用的有价值的工具。
