Abstract：
OBJECTIVE: To assess the impact of various organic and inorganic acids on the roughness, demineralization, and collagen secondary structures of human dentin and to compare these effects with those of traditional agents, specifically phosphoric acid (PA) and ethylenediaminetetraacetic acid (EDTA).
METHODS: Coronal dentin discs (n = 10) were examined by optical profilometry (roughness) and ATR-FTIR before and after conditioning with 32 % PA, 3 % nitric acid (NA), 20 % citric acid (CA), 20 % phytic acid (IP6) or 17 % EDTA. Spectra data were processed to quantify dentin demineralization (DM%) and percentage area of amide I curve-fitted components of β-turns, 310-helix, α-helix, random coils, β-sheets, and collagen maturation index. Statistical analysis was performed by one-way ANOVA or Kruskal-Wallis for DM% and roughness parameters, and paired t-test/Wilcoxon test for amide I components at significance level set at α = 0.05.
RESULTS: All treatments resulted in increased roughness parameters, with the most significant changes occurring primarily with PA, while EDTA exhibited the least changes. DM% was NA>PA>IP6>CA>EDTA in a descending order. Regarding amide I components, NA demonstrated a significant reduction in β-turns, 310-helices, and α-helices and it increased β-sheets and random coils. PA resulted in reduction in β-turns and α-helices while it increased β-sheets. CA and EDTA did not cause significant changes. The collagen maturation index significantly increased only after IP6 treatment.
CONCLUSIONS: The effect on dentin roughness parameters, demineralization, and collagen secondary structures varied based on the type of dentin surface treatment.
CONCLUSIONS: Understanding the impact of acids on the intrinsic properties of dentin is clinically essential for gaining insights into how these effects influence adhesion to dentin, the long-term stability of resin-based restorations, and the success of remineralization therapies.
METHODS: Coronal dentin discs (n = 10) were examined by optical profilometry (roughness) and ATR-FTIR before and after conditioning with 32 % PA, 3 % nitric acid (NA), 20 % citric acid (CA), 20 % phytic acid (IP6) or 17 % EDTA. Spectra data were processed to quantify dentin demineralization (DM%) and percentage area of amide I curve-fitted components of β-turns, 310-helix, α-helix, random coils, β-sheets, and collagen maturation index. Statistical analysis was performed by one-way ANOVA or Kruskal-Wallis for DM% and roughness parameters, and paired t-test/Wilcoxon test for amide I components at significance level set at α = 0.05.
RESULTS: All treatments resulted in increased roughness parameters, with the most significant changes occurring primarily with PA, while EDTA exhibited the least changes. DM% was NA>PA>IP6>CA>EDTA in a descending order. Regarding amide I components, NA demonstrated a significant reduction in β-turns, 310-helices, and α-helices and it increased β-sheets and random coils. PA resulted in reduction in β-turns and α-helices while it increased β-sheets. CA and EDTA did not cause significant changes. The collagen maturation index significantly increased only after IP6 treatment.
CONCLUSIONS: The effect on dentin roughness parameters, demineralization, and collagen secondary structures varied based on the type of dentin surface treatment.
CONCLUSIONS: Understanding the impact of acids on the intrinsic properties of dentin is clinically essential for gaining insights into how these effects influence adhesion to dentin, the long-term stability of resin-based restorations, and the success of remineralization therapies.
摘要：
目的：评估各种有机和无机酸对粗糙度的影响，人牙本质的去矿质和胶原二级结构，并将这些效果与传统药物的效果进行比较，特别是磷酸(PA)和乙二胺四乙酸(EDTA)。
方法：冠状牙本质盘（n=10）通过光学轮廓术（粗糙度）和ATR-FTIR在用32％PA调理之前和之后进行检查，3%硝酸(NA),20%柠檬酸(CA),20%植酸(IP6)或17%EDTA。对光谱数据进行了处理，以量化牙本质脱矿质（DM％）和β转角的酰胺I曲线拟合成分的面积百分比，310螺旋，α-螺旋,随机线圈，β-sheets,和胶原蛋白成熟（R）指数。通过单因素方差分析或Kruskal-Wallis对DM%和粗糙度参数进行统计分析。在α=0.05的显著性水平下，酰胺I成分的配对t检验/Wilcoxon检验。
结果：所有处理都导致粗糙度参数增加，最显著的变化主要发生在PA，而EDTA的变化最小。DM%依次为NA>PA>IP6>CA>EDTA。关于酰胺I组分，NA显示β转角显著减少，310-螺旋α-螺旋和β-折叠和无规卷曲的增加。PA导致β转角减少，α-螺旋和β-折叠的增加，而CA和IP6没有引起显著变化。胶原成熟指数仅随IP6处理而增加。
结论：对牙本质粗糙度参数的影响，脱矿质和胶原二级结构根据牙本质表面处理的类型而变化。
结论：了解酸对牙本质固有特性的影响对于深入了解这些影响如何影响牙本质的粘附在临床上至关重要。树脂基修复体的长期稳定性，和再矿化疗法的成功。
方法：冠状牙本质盘（n=10）通过光学轮廓术（粗糙度）和ATR-FTIR在用32％PA调理之前和之后进行检查，3%硝酸(NA),20%柠檬酸(CA),20%植酸(IP6)或17%EDTA。对光谱数据进行了处理，以量化牙本质脱矿质（DM％）和β转角的酰胺I曲线拟合成分的面积百分比，310螺旋，α-螺旋,随机线圈，β-sheets,和胶原蛋白成熟（R）指数。通过单因素方差分析或Kruskal-Wallis对DM%和粗糙度参数进行统计分析。在α=0.05的显著性水平下，酰胺I成分的配对t检验/Wilcoxon检验。
结果：所有处理都导致粗糙度参数增加，最显著的变化主要发生在PA，而EDTA的变化最小。DM%依次为NA>PA>IP6>CA>EDTA。关于酰胺I组分，NA显示β转角显著减少，310-螺旋α-螺旋和β-折叠和无规卷曲的增加。PA导致β转角减少，α-螺旋和β-折叠的增加，而CA和IP6没有引起显著变化。胶原成熟指数仅随IP6处理而增加。
结论：对牙本质粗糙度参数的影响，脱矿质和胶原二级结构根据牙本质表面处理的类型而变化。
结论：了解酸对牙本质固有特性的影响对于深入了解这些影响如何影响牙本质的粘附在临床上至关重要。树脂基修复体的长期稳定性，和再矿化疗法的成功。
