Abstract：
Chemical investigation of the wild mushroom Entoloma clypeatum led to the isolation of one new A-nor B-aromatic C28 steroid (1), along with eight known compounds (2-9) from this mushroom. As far as we know, compound 1 represents an unprecedented type of natural product. The structure of the new compound was elucidated based on extensive spectroscopic data analysis of HR-ESI-MS, 1D, and 2D NMR, while the relative configuration was confirmed by NOESY correlations. In addition, the anti-inflammatory activity of compound 1 was evaluated against LPS induced NO production in RAW 264.7 macrophages. Compound 1 exhibited a moderate anti-inflammatory activity with an IC50 value of 24.56 ± 1.72 μM.
摘要：
对野生蘑菇Entolomaclypeatum的化学研究导致分离出一种新的A-norB-芳香族C28类固醇（1），以及这种蘑菇中的八种已知化合物（2-9）。据我们所知,化合物1代表了一种前所未有的天然产物。基于HR-ESI-MS的广泛光谱数据分析，阐明了新化合物的结构，1D,和2DNMR，而相对配置由NOESY相关性证实。此外,在RAW264.7巨噬细胞中评价化合物1对LPS诱导的NO产生的抗炎活性。化合物1表现出中等的抗炎活性,IC50值为24.56±1.72μM。
