{Reference Type}: Journal Article
{Title}: Clypeasterol, a novel aromatic ergosterol skeleton from the mushroom Entoloma clypeatum.
{Author}: Lv JH;Yang XM;Xiong MM;Yao L;Wang LA;Li Z;Zhang JX;
{Journal}: Nat Prod Res
{Volume}: 0
{Issue}: 0
{Year}: 2024 Jul 1
{Factor}: 2.488
{DOI}: 10.1080/14786419.2024.2373962
{Abstract}: Chemical investigation of the wild mushroom Entoloma clypeatum led to the isolation of one new A-nor B-aromatic C28 steroid (1), along with eight known compounds (2-9) from this mushroom. As far as we know, compound 1 represents an unprecedented type of natural product. The structure of the new compound was elucidated based on extensive spectroscopic data analysis of HR-ESI-MS, 1D, and 2D NMR, while the relative configuration was confirmed by NOESY correlations. In addition, the anti-inflammatory activity of compound 1 was evaluated against LPS induced NO production in RAW 264.7 macrophages. Compound 1 exhibited a moderate anti-inflammatory activity with an IC50 value of 24.56 ± 1.72 μM.