Abstract：
Investigating the influence of the ambient chemical environment on molecular behaviors in liposomes is crucial for understanding and manipulating cellular vitality as well as the capabilities of lipid drug carriers in various environments. Here, we designed and synthesized a second harmonic generation (SHG) and fluorescence probe molecule called Pyr-Py+-N+ (PPN), which possesses membrane-targeting capability. We employed PPN to investigate the response of lipid vesicles composed of cardiolipin to the presence of exogenous salt. The kinetic behaviors, including the adsorption and embedding of PPN on the surface of small unilamellar vesicles (SUVs) composed of cardiolipin, were analyzed. The response of the SUVs to the addition of NaCl was also monitored. A rapid decrease in vesicle size can be evidenced through the rapid drop in SHG emission originating from PPN located on the vesicle surface.
摘要：
研究环境化学环境对脂质体分子行为的影响对于理解和操纵细胞活力以及脂质药物载体在各种环境中的能力至关重要。这里,我们设计并合成了一种称为Pyr-Py-N（PPN）的二次谐波发生（SHG）和荧光探针分子，具有膜靶向能力。我们使用PPN来研究由心磷脂组成的脂质囊泡对外源盐存在的反应。动力学行为，包括PPN在由心磷脂组成的单层小囊泡（SUV）表面的吸附和包埋，进行了分析。还监测SUV对添加NaCl的响应。囊泡尺寸的快速减小可以通过源自位于囊泡表面上的PPN的SHG发射的快速下降来证明。
