Abstract：
Dengue fever is a mosquito-borne viral disease caused by the dengue virus (DENV). It poses a public health threat globally and, while most people with dengue have mild symptoms or are asymptomatic, approximately 5% of affected individuals develop severe disease and need hospital care. However, knowledge of the molecular mechanisms underlying dengue infection and the interaction between the virus and its host remains limited. In the present study, we performed a quantitative proteomic and N-glycoproteomic analysis of serum from 19 patients with dengue and 11 healthy people. The results revealed distinct proteomic and N-glycoproteomic landscapes between the two groups. Notably, we report for the first time the changes in the serum N glycosylation pattern following dengue infection and provide abundant information on glycoproteins, glycosylation sites, and intact N-glycopeptides using recently developed site-specific glycoproteomic approaches. Furthermore, a series of key functional pathways in proteomic and N-glycoproteomic were identified. Collectively, our findings significantly improve understanding of host and DENV interactions and the general pathogenesis and pathology of DENV, laying a foundation for functional studies of glycosylation and glycan structures in dengue infection.
摘要：
登革热是由登革病毒（DENV）引起的蚊媒病毒性疾病。它在全球范围内构成公共卫生威胁，虽然大多数登革热患者症状轻微或无症状，大约5%的受影响个体发展为严重疾病,需要住院治疗.然而,对登革热感染的分子机制以及病毒与其宿主之间的相互作用的了解仍然有限。在本研究中,我们对19例登革热患者和11例健康人的血清进行了定量蛋白质组学和N-糖蛋白质组学分析.结果揭示了两组之间不同的蛋白质组和N-糖蛋白组景观。值得注意的是,我们首次报道了登革热感染后血清N糖基化模式的变化，并提供了有关糖蛋白的丰富信息，糖基化位点，和完整的N-糖肽使用最近开发的位点特异性糖蛋白质组学方法。此外，鉴定了蛋白质组和N-糖蛋白质组中的一系列关键功能通路。总的来说，我们的发现显着提高了对宿主和DENV相互作用以及DENV的一般发病机制和病理学的理解，为登革热感染中糖基化和聚糖结构的功能研究奠定基础。
