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Abstract：
Kidney clear cell carcinoma (KIRC) commonly presents with metastases upon diagnosis, highlighting the critical need to identify more precise biomarkers for early detection, intervention, and personalized treatment. Although The REEP family has been investigated in cancer development, the specific relationship between REEP4 and cancer remains unclear. In our study, we employed bioinformatics analysis and conducted fundamental experiments to evaluate the potential of REEP4 as a biomarker for predicting the prognosis and therapeutic efficacy of KIRC. Comparing KIRC tumor tissues to normal tissues, we observed a significant upregulation in REEP4 expression, with higher levels of REEP4 correlating positively with tumor malignancy. Further COX regression analysis, as well as single and multifactorial analyses, confirmed that high REEP4 expression indicated lower survival rates in KIRC. Gene function analysis also identified associations between REEP4 and critical pathways such as the cell cycle, along with its involvement in protein binding. Furthermore, our investigation of the immune response suggests that a favorable immunotherapeutic response is linked to a reduction in REEP4 expression. Subsequently, we conducted in vitro experiments to confirm the overexpression of REEP4 in KIRC tumor tissues and renal cancer cells. In summary, our study revealed a close association between REEP4 expression and KIRC, emphasizing its correlation with prognosis and the immune response. These findings suggest that REEP4 is a potential biomarker for KIRC.
摘要：
肾透明细胞癌（KIRC）在诊断时通常会出现转移，强调了识别更精确的生物标志物以进行早期检测的关键需求，干预，个性化治疗。尽管REEP家族已经在癌症发展中进行了调查，REEP4与癌症之间的具体关系尚不清楚.在我们的研究中,我们采用生物信息学分析,并进行了基础实验,以评估REEP4作为预测KIRC预后和疗效的生物标志物的潜力.比较KIRC肿瘤组织与正常组织，我们观察到REEP4表达显著上调，更高水平的REEP4与肿瘤恶性程度呈正相关。进一步的COX回归分析，以及单因素和多因素分析，证实高REEP4表达表明KIRC的存活率较低。基因功能分析还确定了REEP4与细胞周期等关键途径之间的关联。以及它参与蛋白质结合。此外，我们对免疫应答的研究表明,良好的免疫治疗应答与REEP4表达的降低有关.随后，我们进行了体外实验以证实REEP4在KIRC肿瘤组织和肾癌细胞中的过表达。总之,我们的研究表明REEP4表达与KIRC密切相关，强调其与预后和免疫反应的相关性。这些发现表明REEP4是KIRC的潜在生物标志物。
