PDF(Pubmed)
Abstract：
Interstitial fluid (ISF) contains a wealth of biomolecules, yet it is underutilized for diagnostic testing due to a lack of rapid and simple techniques for collecting abundant amounts of fluid. Here, we report a simple and minimally invasive technique for rapidly sampling larger quantities of ISF from human skin. A microneedle array is used to generate micropores in skin from which ISF is extracted using a vacuum-assisted skin patch. Using this technique, an average of 20.8 μL of dermal ISF is collected in 25 min, which is an ∼6-fold improvement over existing sampling methods. Proteomic analysis of collected ISF reveals that it has nearly identical protein composition as blood, and >600 medically relevant biomarkers are identified. Toward this end, we demonstrate the detection of SARS-CoV-2 neutralizing antibodies in ISF collected from COVID-19 vaccinees using two commercial immunoassays, showcasing the utility of this technique for diagnostic testing.
摘要：
间质液（ISF）含有丰富的生物分子，然而，由于缺乏快速和简单的技术来收集大量的液体，它在诊断测试中的应用不足。这里,我们报告了一种简单而微创的技术，可以从人体皮肤中快速采样大量的ISF。微针阵列用于在皮肤中产生微孔,使用真空辅助皮肤贴片从所述微孔中提取ISF。使用这种技术，在25分钟内收集到平均20.8μL的真皮ISF，这比现有的抽样方法提高了约6倍。收集的ISF的蛋白质组学分析显示，它具有与血液几乎相同的蛋白质组成，和>600个医学相关的生物标志物被鉴定。为此，我们证明了使用两种商业免疫测定方法从COVID-19疫苗中收集的ISF中检测到SARS-CoV-2中和抗体，展示了该技术用于诊断测试的实用性。
