{Reference Type}: Journal Article
{Title}: Microneedle-based sampling of dermal interstitial fluid using a vacuum-assisted skin patch.
{Author}: Jiang X;Wilkirson EC;Bailey AO;Russell WK;Lillehoj PB;
{Journal}: Cell Rep Phys Sci
{Volume}: 5
{Issue}: 6
{Year}: 2024 Jun 19
{Factor}: 7.832
{DOI}: 10.1016/j.xcrp.2024.101975
{Abstract}: Interstitial fluid (ISF) contains a wealth of biomolecules, yet it is underutilized for diagnostic testing due to a lack of rapid and simple techniques for collecting abundant amounts of fluid. Here, we report a simple and minimally invasive technique for rapidly sampling larger quantities of ISF from human skin. A microneedle array is used to generate micropores in skin from which ISF is extracted using a vacuum-assisted skin patch. Using this technique, an average of 20.8 μL of dermal ISF is collected in 25 min, which is an ∼6-fold improvement over existing sampling methods. Proteomic analysis of collected ISF reveals that it has nearly identical protein composition as blood, and >600 medically relevant biomarkers are identified. Toward this end, we demonstrate the detection of SARS-CoV-2 neutralizing antibodies in ISF collected from COVID-19 vaccinees using two commercial immunoassays, showcasing the utility of this technique for diagnostic testing.