PDF(Pubmed)
Abstract：
UNASSIGNED: as cholera, due to toxigenic bacteria Vibrio cholera (serogroups O1 and O139), is a major public health threat in Africa, the aim of this work was to investigate potentially pathogenic Vibrionaceae bacteria firstly from human stool samples, and secondly from various environmental water points of Saint-Louis city in Senegal.
UNASSIGNED: a hospital-based study was conducted between 2013 and 2015. Stool samples were taken and cultured from daily incoming patients or hospitalized for acute diarrhea at Saint-Louis´ regional hospital. For environment, a monthly longitudinal sampling from January to October 2016 was carried out at 10 sites in the city. We used total DNA extracted from APW (alkaline peptone water) broth solutions and on suspect bacterial colonies to run PCR Multiplex targeting specific DNA fragments to detect Vibrio genus and specific species. In case of positivity, a simplex PCR was performed to test for cholera toxins Ctx, and V. parahaemolyticus TRH and TDH.
UNASSIGNED: for 43 patients screened, bacterial culture was positive in 6% of cases but no strain of V. cholerae or other Vibrio sp. was isolated. PCR on 90 APW solutions were positive for Vibrio sp.(n = 43), V. cholera(n = 27), V. mimicus(n = 16), V. parahaemolyticus(8), V. alginolyticus(n = 4), and V. vulnificus(n = 2). Unlike for those on suspected colonies which were positive for a majority of V. parahaemolyticus (n = 40) and V. cholerae non-O1 / O139 (n = 35). Six strains of V. parahaemolyticus carried TRH gene, 3 of which expressed simultaneously virulence TRH and TDH genes. For physicochemical parameters, all temperatures varied similarly according to a unimodal seasonality, as well as salinity.
UNASSIGNED: despite the presence of natural populations of Vibrionaceae, even toxigenic ones, was noted in water environment, along with favorable habitat conditions that could play a role in transmission of Vibriosis in the Saint Louis population, we did not isolate any of them from patients screened at the hospital.
UNASSIGNED: a hospital-based study was conducted between 2013 and 2015. Stool samples were taken and cultured from daily incoming patients or hospitalized for acute diarrhea at Saint-Louis´ regional hospital. For environment, a monthly longitudinal sampling from January to October 2016 was carried out at 10 sites in the city. We used total DNA extracted from APW (alkaline peptone water) broth solutions and on suspect bacterial colonies to run PCR Multiplex targeting specific DNA fragments to detect Vibrio genus and specific species. In case of positivity, a simplex PCR was performed to test for cholera toxins Ctx, and V. parahaemolyticus TRH and TDH.
UNASSIGNED: for 43 patients screened, bacterial culture was positive in 6% of cases but no strain of V. cholerae or other Vibrio sp. was isolated. PCR on 90 APW solutions were positive for Vibrio sp.(n = 43), V. cholera(n = 27), V. mimicus(n = 16), V. parahaemolyticus(8), V. alginolyticus(n = 4), and V. vulnificus(n = 2). Unlike for those on suspected colonies which were positive for a majority of V. parahaemolyticus (n = 40) and V. cholerae non-O1 / O139 (n = 35). Six strains of V. parahaemolyticus carried TRH gene, 3 of which expressed simultaneously virulence TRH and TDH genes. For physicochemical parameters, all temperatures varied similarly according to a unimodal seasonality, as well as salinity.
UNASSIGNED: despite the presence of natural populations of Vibrionaceae, even toxigenic ones, was noted in water environment, along with favorable habitat conditions that could play a role in transmission of Vibriosis in the Saint Louis population, we did not isolate any of them from patients screened at the hospital.
摘要：
■作为霍乱，由于产毒细菌霍乱弧菌（血清群O1和O139），是非洲主要的公共卫生威胁，这项工作的目的是首先从人类粪便样本中研究潜在的致病性弧菌科细菌，其次来自塞内加尔圣路易斯市的各种环境用水点。
■一项基于医院的研究于2013年至2015年进行。从每天传入的患者或在圣路易斯地区医院因急性腹泻住院的患者中采集并培养粪便样本。对于环境，2016年1月至10月,在该市10个地点进行了每月纵向采样.我们使用从APW（碱性蛋白胨水）肉汤溶液和可疑细菌菌落中提取的总DNA进行PCRMultiplex靶向特定DNA片段以检测弧菌属和特定物种。在积极的情况下，进行单纯PCR检测霍乱毒素Ctx，以及副溶血性弧菌TRH和TDH。
■对43名患者进行筛查，在6％的病例中，细菌培养呈阳性，但没有霍乱弧菌或其他弧菌。被隔离。90个APW溶液的PCR对弧菌属呈阳性。（n=43），V.霍乱（n=27），V.拟态（n=16），五、副溶血病(8)，V.溶藻(n=4)，和V.创伤（n=2）。与大多数副溶血性弧菌（n=40）和非O1/O139霍乱弧菌（n=35）阳性的可疑菌落不同。6株副溶血性弧菌携带TRH基因,3个同时表达毒力TRH和TDH基因。对于物理化学参数，所有温度根据单峰季节性变化相似，以及盐度。
■尽管存在弧菌科的自然种群，甚至是产毒的，在水环境中被注意到，以及有利的栖息地条件，可以在圣路易斯人群中传播弧菌病，我们没有从医院筛查的患者中分离出任何一种.
■一项基于医院的研究于2013年至2015年进行。从每天传入的患者或在圣路易斯地区医院因急性腹泻住院的患者中采集并培养粪便样本。对于环境，2016年1月至10月,在该市10个地点进行了每月纵向采样.我们使用从APW（碱性蛋白胨水）肉汤溶液和可疑细菌菌落中提取的总DNA进行PCRMultiplex靶向特定DNA片段以检测弧菌属和特定物种。在积极的情况下，进行单纯PCR检测霍乱毒素Ctx，以及副溶血性弧菌TRH和TDH。
■对43名患者进行筛查，在6％的病例中，细菌培养呈阳性，但没有霍乱弧菌或其他弧菌。被隔离。90个APW溶液的PCR对弧菌属呈阳性。（n=43），V.霍乱（n=27），V.拟态（n=16），五、副溶血病(8)，V.溶藻(n=4)，和V.创伤（n=2）。与大多数副溶血性弧菌（n=40）和非O1/O139霍乱弧菌（n=35）阳性的可疑菌落不同。6株副溶血性弧菌携带TRH基因,3个同时表达毒力TRH和TDH基因。对于物理化学参数，所有温度根据单峰季节性变化相似，以及盐度。
■尽管存在弧菌科的自然种群，甚至是产毒的，在水环境中被注意到，以及有利的栖息地条件，可以在圣路易斯人群中传播弧菌病，我们没有从医院筛查的患者中分离出任何一种.
