PDF(Pubmed)
Abstract：
The present study aimed to investigate the effect of catechin-loaded Chitosan-Alginate nanoparticles (NPs) on cognitive function in an aluminum chloride (AlCl3)-induced rat model of Alzheimer\'s disease (AD). The Catechin-loaded Chitosan-Alginate nanocarriers were synthesized through ionotropic gelation (IG) method. Physio-chemical characterization was conducted with the Zetasizer Nano system, the scanning electron microscope, and the Fourier transform infrared spectroscopy. The experiments were performed over 21 days on six groups of male Wistar rats. The control group, AlCl3 treated group, Catechin group, nanocarrier group, treatment group 1 (AlCl3 + Catechin), and treatment group 2 (AlCl3 + nanocarrier). A behavioral study was done by the Morris water maze (MWM) test. In addition, the level of oxidative indices and acetylcholine esterase (AChE) activity was determined by standard procedures at the end of the study. AlCl3 induced a significant increase in AChE activity, along with a significant decrease in the level of Catalase (CAT) and total antioxidant capacity (TAC) in the hippocampus. Moreover, the significant effect of AlCl3 was observed on the behavioral parameters of the MWM test. Both forms of Catechin markedly improved AChE activity, oxidative biomarkers, spatial memory, and learning. The present study indicated that the administration of Catechin-loaded Chitosan-Alginate NPs is a beneficial therapeutic option against behavioral and chemical alteration of AD in male Wistar rats.
摘要：
本研究旨在探讨负载儿茶素的壳聚糖海藻酸盐纳米粒(NPs)对氯化铝(AlCl3)诱导的阿尔茨海默病(AD)大鼠模型认知功能的影响。通过离子凝胶化（IG）方法合成了儿茶素-壳聚糖-海藻酸盐纳米载体。用Zetasizer纳米系统进行了物理化学表征，扫描电子显微镜，和傅里叶变换红外光谱。实验在六组雄性Wistar大鼠上进行21天。对照组,AlCl3治疗组，儿茶素组，纳米载体组，治疗组1（AlCl3+儿茶素），和治疗组2(AlCl3+纳米载体)。通过Morris水迷宫(MWM)测试进行行为研究。此外,在研究结束时通过标准程序确定氧化指数水平和乙酰胆碱酯酶（AChE）活性。AlCl3诱导AChE活性显著增加，海马中过氧化氢酶（CAT）和总抗氧化能力（TAC）的水平显着降低。此外，观察到AlCl3对MWM测试的行为参数的显着影响。两种形式的儿茶素均显着提高了AChE活性，氧化生物标志物，空间记忆,和学习。本研究表明，在雄性Wistar大鼠中，服用载有儿茶素的壳聚糖-海藻酸盐NP是针对AD行为和化学改变的有益治疗选择。
