Abstract：
The aim was to analyse the influence of an apical ledge on root canal disinfection. Forty-four single-rooted teeth were micro-CT scanned and inoculated with Enterococcus faecalis. In Group S shaping was performed with ProTaper Next (PTN) up to X3 at working length (WL). In Group L an apical ledge was created with K-Files #40 and shaping completed up to PTN X3. NaOCl 5% and EDTA 10% irrigant solutions were alternated. Confocal laser scanning microscope (CLSM) and viability staining were used to analyse the proportions of dead (red) and live (green) bacteria and penetration ability inside dentinal tubules. Data were analysed with the Mann-Whitney test with Bonferroni correction (p < 0.05). In Group L the amount of red fluorescence resulted significantly lower, and penetration ability was decreased in the apical and middle portion (p < 0.05). The presence of an apical ledge may negatively influence the disinfection both in the apical and middle third.
摘要：
目的是分析根尖壁架对根管消毒的影响。对44颗单根牙齿进行显微CT扫描，并接种粪肠球菌。在S组中,在工作长度(WL)处用ProTaperNext(PTN)直到X3进行成形。在L组中，使用K-Files＃40创建了根尖壁架，并完成了直到PTNX3的整形。交替使用5%NaOCl和10%EDTA冲洗液。使用共聚焦激光扫描显微镜（CLSM）和活力染色来分析死（红色）和活（绿色）细菌的比例以及牙本质小管内的渗透能力。用具有Bonferroni校正的Mann-Whitney检验分析数据(p<0.05)。在L组中，红色荧光的量显著降低，根尖和中段穿透能力下降(p<0.05)。根尖壁架的存在可能会对根尖和中部三分之一的消毒产生负面影响。
