Abstract：
Oleuropein aglycone (OA), which is the absorbed form of oleuropein, is a major phenolic compound in extra virgin olive oil. We analyzed the anti-obesity effect of OA intake combined with mild treadmill walking (MTW, 4 m/min for 20 min/d, 5-6 d/wk, without electric shocks and slope) in rats under a high-fat diet (HF). Four-week-old male Sprague-Dawley rats (n=28) were equally divided into four groups: control (HF), 0.08% oleuropein-supplemented HF (HFO), HF with MTW (HF+W), and HFO with MTW (HFO+W) groups. After 28 d, the inguinal subcutaneous fat content and weight gain were significantly lower in the HFO+W group than in the control group. The HFO+W group also had significantly higher levels of urinary noradrenaline secretion, interscapular brown adipose tissue, uncoupling protein 1, brain transient receptor potential ankyrin subtype 1 (TRPA1), vanilloid subtype 1 (TRPV1), and brain-derived neurotrophic factor (BDNF) than the control group. Especially, the HFO+W group showed a synergistic effect on noradrenaline secretion. Therefore, OA combined with MTW may accelerate the enhancement of UCP1 and BDNF levels in rats with HF-induced obesity by increasing noradrenaline secretion after TRPA1 and TRPV1 activation.
摘要：
橄榄苦苷苷配基(OA),这是橄榄苦苷的吸收形式，是特级初榨橄榄油中的主要酚类化合物。我们分析了OA摄入联合轻度跑步机步行的抗肥胖作用(MTW，4m/min为20min/d，5-6d/wk,在高脂饮食（HF）下的大鼠中没有电击和斜坡）。将四周大的雄性Sprague-Dawley大鼠（n=28）平均分为四组：对照组（HF），0.08%橄榄苦苷补充HF（HFO），HF与MTW（HF+W），和HFO与MTW(HFO+W)组。28d后，HFO+W组腹股沟皮下脂肪含量和体重增加明显低于对照组。HFO+W组的尿去甲肾上腺素分泌水平也明显较高,肩胛骨间棕色脂肪组织，解偶联蛋白1，脑瞬时受体电位锚蛋白亚型1（TRPA1），香草素亚型1（TRPV1），脑源性神经营养因子(BDNF)高于对照组。尤其是,HFO+W组对去甲肾上腺素分泌有协同作用。因此,OA联合MTW可能通过增加TRPA1和TRPV1激活后去甲肾上腺素的分泌来加速HF诱导肥胖大鼠UCP1和BDNF水平的增强。
