Abstract：
There is an increasing amount of research into the development of a third generation of iron supplementation using peptide-iron chelates. Peptides isolated from mung bean were chelated with ferrous iron (MBP-Fe) and tested as a supplement in mice suffering from iron-deficiency anemia (IDA). Mice were randomly divided into seven groups: a group fed the normal diet, the IDA model group, and IDA groups treated with inorganic iron (FeSO4), organic iron (ferrous bisglycinate, Gly-Fe), low-dose MBP-Fe(L-MBP-Fe), high-dose MBP-Fe(H-MBP-Fe), and MBP mixed with FeSO4 (MBP/Fe). The different iron supplements were fed for 28 days via intragastric administration. The results showed that MBP-Fe and MBP/Fe had ameliorative effects, restoring hemoglobin (HGB), red blood cell (RBC), hematocrit (HCT), and serum iron (SI) levels as well as total iron binding capacity (TIBC) and body weight gain of the IDA mice to normal levels. Compared to the inorganic (FeSO4) and organic (Gly-Fe) iron treatments, the spleen coefficient and damage to liver and spleen tissues were significantly lower in the H-MBP-Fe and MBP/Fe mixture groups, with reparative effects on jejunal tissue. Gene expression analysis of the iron transporters Dmt 1 (Divalent metal transporter 1), Fpn 1 (Ferroportin 1), and Dcytb (Duodenal cytochrome b) indicated that MBP promoted iron uptake. These findings suggest that mung bean peptide-ferrous chelate has potential as a peptide-based dietary supplement for treating iron deficiency.
摘要：
使用肽-铁螯合物开发第三代铁补充剂的研究越来越多。从绿豆中分离的肽与亚铁（MBP-Fe）螯合，并在患有缺铁性贫血（IDA）的小鼠中作为补充剂进行测试。小鼠随机分为七组：一组饲喂正常饮食，IDA模型组，和用无机铁(FeSO4)处理的IDA组，有机铁（双甘氨酸亚铁，Gly-Fe),低剂量MBP-Fe(L-MBP-Fe),高剂量MBP-Fe(H-MBP-Fe),和与FeSO4混合的MBP(MBP/Fe)。不同的铁补充剂通过胃内给药喂养28天。结果表明，MBP-Fe和MBP/Fe具有改善作用，恢复血红蛋白（HGB），红细胞（RBC），血细胞比容(HCT),和血清铁（SI）水平以及总铁结合能力（TIBC）和IDA小鼠的体重增加至正常水平。与无机（FeSO4）和有机（Gly-Fe）铁处理相比，H-MBP-Fe和MBP/Fe混合物组的脾脏系数和对肝脏和脾脏组织的损伤程度明显降低，对空肠组织有修复作用。铁转运蛋白Dmt1（二价金属转运蛋白1）的基因表达分析，Fpn1(Ferroportin1),Dcytb(十二指肠细胞色素b)表明MBP促进铁摄取。这些发现表明绿豆肽-亚铁螯合物具有作为治疗铁缺乏的基于肽的膳食补充剂的潜力。
