Abstract:
Respirometry is an important tool for understanding whole-animal energy and water balance in relation to the environment. Consequently, the growing number of studies using respirometry over the last decade warrants reliable reporting and data sharing for effective dissemination and research synthesis. We provide a checklist guideline on five key sections to facilitate the transparency, reproducibility, and replicability of respirometry studies: 1) materials, set up, plumbing, 2) subject conditions/maintenance, 3) measurement conditions, 4) data processing, and 5) data reporting and statistics, each with explanations and example studies. Transparency in reporting and data availability has benefits on multiple fronts. Authors can use this checklist to design and report on their study, and reviewers and editors can use the checklist to assess the reporting quality of the manuscripts they review. Improved standards for reporting will enhance the value of primary studies and will greatly facilitate the ability to carry out higher quality research syntheses to address ecological and evolutionary theories.
摘要:
呼吸测定法是了解整个动物能量和水平衡与环境关系的重要工具。因此,在过去十年中,使用呼吸测量法的研究越来越多,因此需要可靠的报告和数据共享,以进行有效的传播和研究综合。我们提供了五个关键部分的清单指南,以促进透明度,再现性,呼吸测定研究的可重复性:1)材料,设置,管道,2)主题条件/维护,3)测量条件,4)数据处理,5)数据报告和统计,每个都有解释和例子研究。报告和数据可用性的透明度在多个方面都有好处。作者可以使用这个清单来设计和报告他们的研究,审稿人和编辑可以使用清单来评估他们审稿的报告质量。改进的报告标准将提高初级研究的价值,并将极大地促进开展更高质量的研究综合以解决生态和进化理论的能力。