Abstract：
Antimicrobial treatment of methicillin-resistant Staphylococcus pseudintermedius associated with canine wounds represents an important challenge. The aim of this study was to create a canine wound infection model, Lubbock Chronic Wound Biofilm (LCWB), with a focus on S. pseudintermedius, drawing inspiration from the established human model involving S. aureus. Methicillin-resistant S. pseudintermedius 115 (MRSP) and Pseudomonas aeruginosa 700 strains, isolated from dog wounds, were used to set up the LCWB at 24, 48 and 72h. The LCWBs were evaluated in terms of volume, weight, and microbial CFU/mg. The microbial spatial distribution in the LCWBs was assessed by SEM and CLSM imaging. The best incubation time for the LCWB production in terms of volume (3.38 cm3 ± 0.13), weight (0.86 gr ± 0.02) and CFU/mg (up to 7.05 x 106 CFU/mg ± 2.89 x 105) was 48h. The SEM and CLSM images showed a major viable microbial colonization at 48h with a non-mixed bacteria with a prevalence of MRSP on the surface and P. aeruginosa 700 in the depth of the wound. The obtained findings demonstrate the capability of S. pseudintermedius to grow together P. aeruginosa in the LCWB model, representing the suitable model to reproduce the animal chronic wound in vitro.
摘要：
与犬伤口相关的耐甲氧西林假中间体葡萄球菌的抗菌治疗是一项重要挑战。本研究的目的是建立犬伤口感染模型，Lubbock慢性伤口生物膜（LCWB），专注于假中介，从建立的涉及金黄色葡萄球菌的人体模型中汲取灵感。耐甲氧西林假中间芽孢杆菌115（MRSP）和铜绿假单胞菌700株，与狗的伤口隔离，分别用于在24、48和72小时设置LCWB。LCWB在体积方面进行了评估，体重,和微生物CFU/mg。通过SEM和CLSM成像评估LCWB中的微生物空间分布。LCWB生产的最佳孵育时间（3.38cm3±0.13），体重（0.86gr±0.02）和CFU/mg（最高为7.05x106CFU/mg±2.89x105）为48h。SEM和CLSM图像显示在48小时时主要的有活力的微生物定植,其中非混合细菌在表面上具有MRSP的普遍性,并且在伤口的深度中具有铜绿假单胞菌700的普遍性。获得的研究结果表明，假中间菌在LCWB模型中共同生长铜绿假单胞菌的能力，代表了体外复制动物慢性伤口的合适模型。
