Abstract：
Brown adipose tissue (BAT) is mitochondria rich, enabling high oxidative metabolism for non-shivering thermogenesis. The release of large/small extracellular vesicles (EVs) containing mitochondria or mitochondrial fragments, termed mito-EVs, may support mitochondrial quality control or intercellular communication. We present a protocol to isolate and characterize mito-EVs. We detail steps for BAT processing, cell debris removal, differential centrifugation (dC), and mito-EV analysis by flow cytometry and immunoblotting assays. For complete details on the use and execution of this protocol, please refer to Rosina et al.1.
摘要：
棕色脂肪组织（BAT）富含线粒体，使非发抖产热的高氧化代谢。释放含有线粒体或线粒体片段的大/小细胞外囊泡（EV），被称为mito-EV，可能支持线粒体质量控制或细胞间通讯。我们提出了一种分离和表征mito-EV的方案。我们详细介绍了BAT处理的步骤，细胞碎片去除，差速离心(dC)，并通过流式细胞术和免疫印迹分析进行mito-EV分析。有关此协议的使用和执行的完整详细信息，PleaserefertoRosinaetal.1.
