%0 Journal Article
%T Protocol for detecting mitochondria extracellular vesicles of brown adipose tissue in mice.
%A Tortolici F
%A Di Biagio C
%A Lettieri-Barbato D
%A Aquilano K
%J STAR Protoc
%V 5
%N 3
%D 2024 Jun 27
%M 38943650
暂无%R 10.1016/j.xpro.2024.103161
%X Brown adipose tissue (BAT) is mitochondria rich, enabling high oxidative metabolism for non-shivering thermogenesis. The release of large/small extracellular vesicles (EVs) containing mitochondria or mitochondrial fragments, termed mito-EVs, may support mitochondrial quality control or intercellular communication. We present a protocol to isolate and characterize mito-EVs. We detail steps for BAT processing, cell debris removal, differential centrifugation (dC), and mito-EV analysis by flow cytometry and immunoblotting assays. For complete details on the use and execution of this protocol, please refer to Rosina et al.1.