PDF(Pubmed)
Abstract：
The fish disease model facilitates our understanding of disease dynamics, risk assessment for disease outbreaks, the response of the gut immune system, and the maintenance of ecosystem health. Here, we present a protocol for studying gut immunity modulation by infecting Lepidocephalichthys guntea, a loach fish, with Aeromonas hydrophila. We describe steps for performing intra-peritoneal injection on fish and a bath challenge. We detail procedures for conducting periodic population calculations during the infection phase to corroborate Aeromonas hydrophila invasion in the gut. For complete details on the use and execution of this protocol, please refer to Basak and Chakraborty.1.
摘要：
鱼类疾病模型有助于我们对疾病动力学的理解，疾病爆发的风险评估，肠道免疫系统的反应，以及维持生态系统健康。这里,我们提出了一种通过感染Lepidocephalichysguntea来研究肠道免疫调节的方案，一条泥炭鱼,嗜水气单胞菌.我们描述了对鱼进行腹膜内注射和沐浴挑战的步骤。我们详细介绍了在感染阶段进行定期种群计算的程序，以证实嗜水气单胞菌在肠道中的入侵。有关此协议的使用和执行的完整详细信息，请参考Basak和Chakraborty.1。
