Abstract：
Omega-3 long-chain polyunsaturated fatty acids (LCPUFA) play critical roles in human development and health. Their intake is often effectively estimated solely based on seafood consumption, though the high intake of terrestrial animal-based foods with minor amounts of LCPUFA may be significant. Covalent adduct chemical ionization (CACI) tandem mass spectrometry is one approach for de novo structural and quantitative analysis of minor unsaturated fatty acids (FA), for which standards are unavailable. Here, CACI-MS and MS/MS are used to identify and quantify minor omega-3 LCPUFA of terrestrial animal foods based on the application of measured response factors (RFs) to various FA. American mean intakes of pork, beef, chicken, and eggs contribute 20, 27, 45, and 71 mg/day of docosahexaenoic acid (DHA), respectively. The estimated intake of omega-3 DHA, eicosapentaenoic acid, and docosapentaenoic acid from nonseafood sources is significant, at 164, 103, and 330 mg/day, greater than most existing estimates of omega-3 LCPUFA intake.
摘要：
Omega-3长链多不饱和脂肪酸（LCPUFA）在人类发育和健康中起着至关重要的作用。它们的摄入量通常仅根据海鲜消费来有效估计，尽管少量LCPUFA的陆生动物性食物的高摄入量可能很重要。共价加合物化学电离（CACI）串联质谱是一种从头结构和定量分析次要不饱和脂肪酸（FA）的方法，对于哪些标准是不可用的。这里,CACI-MS和MS/MS用于根据测量的响应因子（RF）对各种FA的应用来鉴定和定量陆生动物食品的次要omega-3LCPUFA。美国人平均猪肉摄入量，牛肉,鸡肉,鸡蛋贡献20、27、45和71毫克/天的二十二碳六烯酸（DHA），分别。omega-3DHA的估计摄入量，二十碳五烯酸,来自非海鲜来源的二十二碳五烯酸是重要的，164、103和330毫克/天，高于大多数现有的omega-3LCPUFA摄入量估计值。
