Abstract：
A strategy for vaccine design involves identifying proteins that could be involved in pathogen-host interactions. The aim of this proteomic study was to determine how iron limitation affects the protein expression of Tenacibaculum dicentrarchi, with a primary focus on virulence factors and proteins associated with iron uptake. The proteomic analysis was carried out using two strains of T. dicentrarchi grown under normal (control) and iron-limited conditions, mimicking the host environment. Our findings revealed differences in the proteins expressed by the type strain CECT 7612T and the Chilean strain TdCh05 of T. dicentrarchi. Nonetheless, both share a common response to iron deprivation, with an increased expression of proteins associated with iron oxidation and reduction metabolism (e.g., SufA, YpmQ, SufD), siderophore transport (e.g., ExbD, TonB-dependent receptor, HbpA), heme compound biosynthesis, and iron transporters under iron limitation. Proteins involved in gliding motility, such as GldL and SprE, were also upregulated in both strains. A negative differential regulation of metabolic proteins, particularly those associated with amino acid biosynthesis, was observed under iron limitation, reflecting the impact of iron availability on bacterial metabolism. Additionally, the TdCh05 strain exhibited unique proteins associated with gliding motility machinery and phage infection control compared to the type strain. These groups of proteins have been identified as virulence factors within the Flavobacteriaceae family, including the genus Tenacibaculum. These results build upon our previous report on iron acquisition mechanisms and could lay the groundwork for future studies aimed at elucidating the role of some of the described proteins in the infectious process of tenacibaculosis, as well as in the development of potential vaccines.
摘要：
疫苗设计的策略涉及鉴定可能参与病原体-宿主相互作用的蛋白质。这项蛋白质组学研究的目的是确定铁限制如何影响双头tenacibaculum的蛋白质表达，主要关注与铁吸收相关的毒力因子和蛋白质。使用在正常（对照）和铁限制条件下生长的两种T.dicentrarchi菌株进行蛋白质组学分析，模仿主机环境。我们的发现揭示了T.dicentrarchi的CECT7612T型菌株和智利菌株TdCh05表达的蛋白质存在差异。尽管如此，两者都对铁剥夺有共同的反应，与铁氧化和还原代谢相关的蛋白质表达增加(例如，Sufa,YpmQ,SufD),铁载体运输(例如，ExbD,TonB依赖性受体，HbpA),血红素化合物生物合成，和铁限制下的铁转运者。参与滑翔运动的蛋白质，比如GldL和SprE,在两个菌株中也上调。代谢蛋白质的负差异调节，特别是那些与氨基酸生物合成有关的，在铁限制下观察到，反映了铁的可用性对细菌代谢的影响。此外,与类型菌株相比，TdCh05菌株表现出与滑动运动机制和噬菌体感染控制相关的独特蛋白质。这些蛋白质组已被鉴定为黄杆菌科的毒力因子，包括Tenacibaculum属。这些结果建立在我们先前关于铁获取机制的报告的基础上，可以为将来的研究奠定基础，旨在阐明某些所述蛋白质在tenacibaculosis感染过程中的作用。以及潜在疫苗的开发。
