Abstract：
UNASSIGNED: The formation of biofilms, characterized by cell aggregation and extracellular polymeric substance (EPS) production, is a common feature of periprosthetic joint infections (PJI).
UNASSIGNED: The current study aimed to investigate the development of biofilm features in vitro within less than 3 weeks by Staphylococcus aureus isolated from PJIs.
UNASSIGNED: Biofilms were grown on sandblasted titanium discs, and fluorescence spectroscopy and microscopy were used to observe biofilm maturation for 21 days.
UNASSIGNED: DNA mass decreased initially, then increased from day 5 onwards, and decreased again after day 7. The proportion of living to dead bacteria oscillated until day 7 and increased at day 10 for strain A and day 14 for strain B. EPS mass decreased initially and then continuously increased. Multilayer bacterial organization was observed at day 7.
UNASSIGNED: Cell aggregation occurred during the first week, followed by EPS production in the second week, and characteristic biofilm features were observed within 1 to 2 weeks.
UNASSIGNED: The current study aimed to investigate the development of biofilm features in vitro within less than 3 weeks by Staphylococcus aureus isolated from PJIs.
UNASSIGNED: Biofilms were grown on sandblasted titanium discs, and fluorescence spectroscopy and microscopy were used to observe biofilm maturation for 21 days.
UNASSIGNED: DNA mass decreased initially, then increased from day 5 onwards, and decreased again after day 7. The proportion of living to dead bacteria oscillated until day 7 and increased at day 10 for strain A and day 14 for strain B. EPS mass decreased initially and then continuously increased. Multilayer bacterial organization was observed at day 7.
UNASSIGNED: Cell aggregation occurred during the first week, followed by EPS production in the second week, and characteristic biofilm features were observed within 1 to 2 weeks.
摘要：
■生物膜的形成，以细胞聚集和胞外聚合物（EPS）产生为特征，是关节假体周围感染（PJI）的常见特征。
■本研究旨在研究从PJIs分离的金黄色葡萄球菌在不到3周内的体外生物膜特征的发展。
■生物膜生长在喷砂钛圆盘上，荧光光谱和显微镜观察生物膜成熟21天。
■DNA质量最初减少，然后从第五天开始增加，并在第7天后再次下降。活细菌与死细菌的比例振荡直到第7天，菌株A在第10天增加，菌株B在第14天增加。EPS质量最初降低，然后连续增加。在第7天观察到多层细菌组织。
■细胞聚集发生在第一周，其次是第二周的每股收益产量，并在1~2周内观察到特征性生物膜特征。
■本研究旨在研究从PJIs分离的金黄色葡萄球菌在不到3周内的体外生物膜特征的发展。
■生物膜生长在喷砂钛圆盘上，荧光光谱和显微镜观察生物膜成熟21天。
■DNA质量最初减少，然后从第五天开始增加，并在第7天后再次下降。活细菌与死细菌的比例振荡直到第7天，菌株A在第10天增加，菌株B在第14天增加。EPS质量最初降低，然后连续增加。在第7天观察到多层细菌组织。
■细胞聚集发生在第一周，其次是第二周的每股收益产量，并在1~2周内观察到特征性生物膜特征。
