%0 Journal Article
%T S. aureus develops biofilms on titanium within 2 weeks in vitro.
%A von Hertzberg-Boelch SP
%A Rudert M
%A Haker F
%A Groll J
%A Ewald A
%J Technol Health Care
%V 0
%N 0
%D 2024 Jun 5
%M 38943411
%F 1.205
%R 10.3233/THC-232041
%X <B>UNASSIGNED: </B>The formation of biofilms, characterized by cell aggregation and extracellular polymeric substance (EPS) production, is a common feature of periprosthetic joint infections (PJI).<BR><B>UNASSIGNED: </B>The current study aimed to investigate the development of biofilm features in vitro within less than 3 weeks by Staphylococcus aureus isolated from PJIs.<BR><B>UNASSIGNED: </B>Biofilms were grown on sandblasted titanium discs, and fluorescence spectroscopy and microscopy were used to observe biofilm maturation for 21 days.<BR><B>UNASSIGNED: </B>DNA mass decreased initially, then increased from day 5 onwards, and decreased again after day 7. The proportion of living to dead bacteria oscillated until day 7 and increased at day 10 for strain A and day 14 for strain B. EPS mass decreased initially and then continuously increased. Multilayer bacterial organization was observed at day 7.<BR><B>UNASSIGNED: </B>Cell aggregation occurred during the first week, followed by EPS production in the second week, and characteristic biofilm features were observed within 1 to 2 weeks.