Abstract：
Large terpene synthases (large-TSs) are a new family of TSs. The first large-TS discovered was from Bacillus subtilis (BsuTS), which is involved in the biosynthesis of a C35 sesquarterpene. Large-TSs are the only enzymes that enable the biosynthesis of sesquarterpenes and do not share any sequence homology with canonical Class I and II TSs. Thus, the investigation of large-TSs is promising for expanding the chemical space in the terpene field. In this chapter, we describe the experimental methods used for identifying large-TSs, as well as their functional and structural analyses. Additionally, several enzymes related to the biosynthesis of large-TS substrates have been described.
摘要：
大萜合酶(large-TS)是新的TS家族。发现的第一个大型TS来自枯草芽孢杆菌（BsuTS），它参与了C35倍半萜的生物合成。大TS是仅有的能够生物合成芝麻的酶，并且与规范的I类和II类TS不具有任何序列同源性。因此，大TSs的研究有望扩大萜烯领域的化学空间。在这一章中,我们描述了用于识别大型TS的实验方法，以及它们的功能和结构分析。此外,已经描述了几种与大TS底物的生物合成有关的酶。
