Abstract：
Alzheimer\'s disease (AD), the most frequent neurodegenerative disease within dementias, affects the CNS, leading to gradual memory issues and cognitive dysfunction. Oxidative stress in AD contributes to ongoing neuronal loss and hastens disease progression. Notably, the potent antioxidant compounds morin and hesperidin have demonstrated significant effectiveness in addressing oxidative stress. This study explores the impact of morin and hesperidin on behavior and oxidative stress in the streptozotocin (STZ)-induced AD rat model. The experiment involved five groups: control, STZ, STZ+morin, STZ+hesperidin, and STZ+morin+hesperidin. The rat model of AD was created by injecting STZ with the stereotaxic surgery. Morin and hesperidin were applied to the groups for 7-days. After the applications, the Morris water maze (MWM) and novel object recognition (NOR) tests were used and the rats were sacrificed. Malondialdehyde (MDA), glutathione (GSH), nitric oxide (NOx), and protein carbonyl (PC) levels were measured. In the STZ group, the levels of NOx and PC exhibited a noteworthy increase compared to the control. Conversely, the application of morin and/or hesperidin treatments reduced NOx and PC levels compared to the STZ group. The co-administration of morin and hesperidin improved the antioxidant status and decreased lipid peroxidation in STZ-induced rats. In the STZ group, serum advanced oxidation protein products (AOPP) levels were statistically elevated compared to the control. However, in the treatment groups, morin and/or hesperidin successfully decreased AOPP levels to those observed in the control. The combined use of these flavonoids may have a neuroprotective effect regarding memory problems and decreasing oxidative/nitrosative stress.
摘要：
阿尔茨海默病（AD），痴呆症中最常见的神经退行性疾病，影响中枢神经系统，导致逐渐的记忆问题和认知功能障碍。AD中的氧化应激有助于持续的神经元损失并加速疾病进展。值得注意的是,有效的抗氧化化合物莫林和橙皮苷已证明在解决氧化应激方面具有显著的有效性。本研究探讨了桑色素和橙皮苷对链脲佐菌素（STZ）诱导的AD大鼠模型行为和氧化应激的影响。实验涉及五组：对照组，STZ,STZ+Morin,STZ+橙皮苷,和STZ+莫林+橙皮苷。通过立体定向手术注射STZ来创建AD的大鼠模型。将莫林和橙皮苷应用于该组7天。在申请之后，使用Morris水迷宫（MWM）和新物体识别（NOR）测试并处死大鼠。丙二醛(MDA),谷胱甘肽(GSH),一氧化氮（NOx），测定蛋白质羰基(PC)水平。在STZ组中,与对照相比,NOx和PC的水平表现出显著的增加。相反，与STZ组相比,应用莫林和/或橙皮苷处理降低了NOx和PC水平。sorin和橙皮苷的共同给药改善了STZ诱导的大鼠的抗氧化状态并降低了脂质过氧化。在STZ组中,与对照组相比，血清高级氧化蛋白产物（AOPP）水平在统计学上升高。然而,在治疗组中，莫林和/或橙皮苷成功地将AOPP水平降低到对照中观察到的水平。这些类黄酮的组合使用可能对记忆问题和减少氧化/亚硝基应激具有神经保护作用。
