Abstract：
Fecal metaproteomics is a useful approach to measure changes in microbial and host protein abundance and to infer which members of the gut microbiota are involved in specific functions and pathways. This chapter describes a protocol enabling analysis and characterization of fecal metaproteomes, successfully applied to human, mouse, and rat stool samples. The protocol combines mechanical and thermal treatments for protein extraction, a centrifugal filter-based procedure for cleanup and digestion, long-gradient liquid chromatography for peptide separation, and high-resolution mass spectrometry for peptide detection.
摘要：
粪便元蛋白质组学是测量微生物和宿主蛋白质丰度变化并推断肠道微生物群的哪些成员参与特定功能和途径的有用方法。本章描述了一个方案，能够分析和表征粪便元蛋白质组，成功应用于人类，鼠标,和大鼠粪便样本。该协议结合了蛋白质提取的机械和热处理，基于离心过滤器的清理和消化程序，用于肽分离的长梯度液相色谱，和用于肽检测的高分辨率质谱。
