Abstract：
Metaproteomics represents a promising and fast method to analyze the taxonomic and functional composition of biogas plant microbiomes. However, metaproteomics sample preparation and bioinformatics analysis is still challenging due to the sample complexity and contaminants. In this chapter, a tailored workflow including sampling, phenol extraction in a ball mill, amido black protein quantification, FASP digestion, LC-MS/MS measurement as well as bioinformatics and biostatistical data evaluation are here described for the metaproteomics advancements applied to biogas plant samples.
摘要：
元蛋白质组学代表了一种有前途的快速方法来分析沼气植物微生物群的分类和功能组成。然而,由于样品的复杂性和污染物，超蛋白质组学样品制备和生物信息学分析仍然具有挑战性。在这一章中,量身定制的工作流程，包括采样，苯酚在球磨机中提取，氨基黑蛋白定量，FASP消化，本文描述了LC-MS/MS测量以及生物信息学和生物统计数据评估，用于应用于沼气植物样品的元蛋白质组学进展。
