Abstract：
The metaproteomic approach allows a deep microbiome characterization in different complex systems. Based on metaproteome data, microbial communities\' composition, succession, and functional role in different environmental conditions can be established.The main challenge in metaproteomic studies is protein extraction, and although many protocols have been developed, a few are focused on the protein extraction of fermented foods. In this chapter, a reproducible and efficient method for the extraction of proteins from a traditionally fermented starchy food is described. The method can be applied to any fermented food and aims to enrich the extraction of proteins from microorganisms for their subsequent characterization.
摘要：
元蛋白质组学方法允许在不同复杂系统中进行深层微生物组表征。根据元蛋白质组数据，微生物群落组成，继承,在不同环境条件下的功能作用可以建立。元蛋白质组学研究的主要挑战是蛋白质提取，尽管已经开发了许多协议，一些是专注于发酵食品的蛋白质提取。在这一章中,描述了一种从传统发酵的淀粉食品中提取蛋白质的可重复且有效的方法。该方法可应用于任何发酵食品，旨在丰富从微生物中提取的蛋白质用于随后的表征。
