Abstract：
As a protein structurally similar to insulin, relaxin3 (RLN3) plays a role in promoting arousal, suppressing depressive or anxious behaviors. Two studies revealed the increase of RLN3 expression during chicken follicle selection. In this study, by real-time quantitative PCR and luciferase assay, mRNA expression and single nucleotide polymorphisms (SNPs) of chicken RLN3 were investigated. The mRNA expression of chicken RLN3 was higher in the granulosa cell of hierarchal follicles (Post-GCs) than that of pre-hierarchal follicles (Pre-GCs). In Pre-GCs, the mRNA expression of chicken RLN3 was stimulated by FSH and progesterone; in Post-GCs, it was stimulated by higher concentration of estrogen and FSH, however, was inhibited by progesterone. Four SNPs including g.-655G > C, g-592G > A, g.-372T > A and g.-282G > C were identified in the critical promoter region from -1291 bp to -207 bp of chicken RLN3, among which g.-655G > C, and g-592G > A were associated with age at first laying and clutch size, respectively, in Zaozhuang Sunzhi chickens. At g.-655G > C and g-592G > A, allele C and allele A had higher transcriptional activity, respectively. These data suggest that RLN3 plays an important role in chicken follicle development and SNPs in its promoter region are potential DNA markers for improving egg production traits.
摘要：
作为一种结构上类似于胰岛素的蛋白质，relaxin3（RLN3）在促进唤醒中起作用，抑制抑郁或焦虑行为。两项研究揭示了鸡卵泡选择过程中RLN3表达的增加。在这项研究中,通过实时定量PCR和荧光素酶分析，研究了鸡RLN3的mRNA表达和单核苷酸多态性（SNP）。鸡RLN3的mRNA在分级卵泡（Post-GCs）的颗粒细胞中的表达高于分级卵泡（Pre-GCs）。在前GC中，FSH和孕酮刺激鸡RLN3的mRNA表达；在GCs后，它受到较高浓度的雌激素和FSH的刺激，然而,被孕酮抑制。四个SNP，包括g.-655G>C，g-592G>A,在鸡RLN3的-1291bp至-207bp的关键启动子区域中鉴定出g.-372T>A和g.-282G>C，其中g.-65G>C，g-592G>A与初次铺设时的年龄和离合器尺寸有关，分别,在枣庄孙芝鸡。在g.-655G>C和g-592G>A时，等位基因C和等位基因A具有较高的转录活性,分别。这些数据表明RLN3在鸡卵泡发育中起重要作用，其启动子区域的SNP是改善产蛋性状的潜在DNA标记。
