Abstract：
OBJECTIVE: Ultraviolet light in the UV-C band is known as germicidal radiation and was widely used for both sterilization of the equipment and creation of a sterile environment. The aim of the study is to assess the effectiveness of inactivation of microorganisms deposited on surfaces with various textures by UV-C radiation disinfection devices.
METHODS: Five microorganisms (3 bacteria, virus, and fungus) deposited on metal, plastic, and glass surfaces with smooth and rough textures were irradiated with UV-C light emitted by low-pressure mercury lamp and ultraviolet emitting diodes (LEDs), from a distance of 0.5 m, 1 m, and 1.5 m to check their survivability after 20-minute exposure.
CONCLUSIONS: Both tested UV-C sources were effective in inactivation of microorganisms; however, LED emitter was more efficient in this respect than the mercury lamp. The survival rate of microorganisms depended on the UV-C dose, conditioned by the distance from UV-C source being the highest at 0.5 m and the lowest at 1.5 m. For the tested microorganisms, the highest survival rate after UV-C irradiation was usually visible on glass and plastic surfaces. This observation should be considered in all environments where the type of material (from which the elements of technical equipment are manufactured and may be contaminated by specific activities) is important for maintaining the proper level of hygiene and avoiding the unwanted and uncontrolled spread of microbiological pollution.
METHODS: Five microorganisms (3 bacteria, virus, and fungus) deposited on metal, plastic, and glass surfaces with smooth and rough textures were irradiated with UV-C light emitted by low-pressure mercury lamp and ultraviolet emitting diodes (LEDs), from a distance of 0.5 m, 1 m, and 1.5 m to check their survivability after 20-minute exposure.
CONCLUSIONS: Both tested UV-C sources were effective in inactivation of microorganisms; however, LED emitter was more efficient in this respect than the mercury lamp. The survival rate of microorganisms depended on the UV-C dose, conditioned by the distance from UV-C source being the highest at 0.5 m and the lowest at 1.5 m. For the tested microorganisms, the highest survival rate after UV-C irradiation was usually visible on glass and plastic surfaces. This observation should be considered in all environments where the type of material (from which the elements of technical equipment are manufactured and may be contaminated by specific activities) is important for maintaining the proper level of hygiene and avoiding the unwanted and uncontrolled spread of microbiological pollution.
摘要：
目的：UV-C波段的紫外线被称为杀菌辐射，广泛用于设备的灭菌和无菌环境的创建。该研究的目的是评估通过UV-C辐射消毒装置灭活沉积在具有各种纹理的表面上的微生物的有效性。
方法：五种微生物（3种细菌，病毒,和真菌）沉积在金属上，塑料,用低压汞灯和紫外发光二极管（LED）发出的UV-C光照射具有光滑和粗糙纹理的玻璃表面，距离0.5米，1米,和1.5m以检查其暴露20分钟后的生存能力。
结论：两种测试的UV-C源都能有效灭活微生物；然而，LED发射器在这方面比汞灯更有效。微生物的存活率取决于UV-C剂量,条件是与UV-C源的距离最高为0.5m，最低为1.5m。对于测试的微生物，UV-C照射后的最高存活率通常在玻璃和塑料表面上可见。在材料类型（制造技术设备的元件并可能受到特定活动污染）对于保持适当的卫生水平和避免不必要的和不受控制的微生物污染的所有环境中，都应考虑这一观察结果。
方法：五种微生物（3种细菌，病毒,和真菌）沉积在金属上，塑料,用低压汞灯和紫外发光二极管（LED）发出的UV-C光照射具有光滑和粗糙纹理的玻璃表面，距离0.5米，1米,和1.5m以检查其暴露20分钟后的生存能力。
结论：两种测试的UV-C源都能有效灭活微生物；然而，LED发射器在这方面比汞灯更有效。微生物的存活率取决于UV-C剂量,条件是与UV-C源的距离最高为0.5m，最低为1.5m。对于测试的微生物，UV-C照射后的最高存活率通常在玻璃和塑料表面上可见。在材料类型（制造技术设备的元件并可能受到特定活动污染）对于保持适当的卫生水平和避免不必要的和不受控制的微生物污染的所有环境中，都应考虑这一观察结果。
