PDF(Pubmed)
Abstract：
The standardization of clinical studies using extracellular vesicles (EVs) has mainly focused on the procedures employed for their isolation and characterization; however, preanalytical aspects of sample collection, handling and storage also significantly impact the reproducibility of results. We conducted an online survey based on SPREC (Standard PREanalytical Code) among members of GEIVEX (Grupo Español de Investigación en Vesiculas Extracelulares) to explore how different laboratories handled fluid biospecimens destined for EV analyses. We received 70 surveys from forty-three different laboratories: 44% focused on plasma, 9% on serum and 16% on urine. The survey indicated that variability in preanalytical approaches reaches 94%. Moreover, in some cases, researchers had no access to all relevant preanalytical details of samples, with some sample aspects with potential impact on EV isolation/characterisation not coded within the current version of SPREC. Our study highlights the importance of working with common standard operating procedures (SOP) to control preanalytical conditions. The application of SPREC represents a suitable approach to codify and register preanalytical conditions. Integrating SPREC into the SOPs of laboratories/biobanks will provide a valuable source of information and constitute an advance for EV research by improving reproducibility and credibility.
摘要：
使用细胞外囊泡（EV）的临床研究的标准化主要集中在分离和表征所采用的程序;然而，样品收集的分析前方面，处理和储存也显著影响结果的可重复性。我们在GEIVEX（GrupoEspañoldeInvestigaciónVesiculasExtracelulares）成员中进行了基于SPREC（标准预分析代码）的在线调查，以探讨不同实验室如何处理用于EV分析的流体生物标本。我们收到了来自43个不同实验室的70项调查：44%的调查集中在血浆上，9%的血清和16%的尿液。调查表明，分析前方法的变异性达到94％。此外，在某些情况下,研究人员无法获得样品的所有相关分析前细节，某些样本方面对EV隔离/表征有潜在影响，但未在当前版本的SPREC中编码。我们的研究强调了使用通用标准操作程序（SOP）控制分析前条件的重要性。SPREC的应用代表了一种编码和配准分析前条件的合适方法。将SPREC集成到实验室/生物库的SOP中，将提供有价值的信息来源，并通过提高可重复性和可信性，为EV研究提供了进步。
