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Abstract：
Fungal polysaccharides are commonly utilized in the food industry and biomedical fields as a natural and safe immune modulator. Co-culturing is a valuable method for enhancing the production of secondary metabolites. This study used intracellular polysaccharide (IPS) content as a screening index, co-culturing seven different fungi with Sanghuangporus vaninii. The seed pre-culture liquid culture time was selected through screening, and conditions were assessed using single factor experimentation, a Plackett-Burman (PB) design, and response surface methodology (RSM) optimization. RSM optimization was conducted, leading to the measurement of antioxidant capacity. Results indicated that the co-culture of S. vaninii and Pleurotus sapidus exhibited the most effective outcome. Specifically, pre-culturing S. vaninii and P. sapidus seed cultures for 2 days and 0 days, respectively, followed by co-culturing, significantly increased IPS content compared to single-strain culturing. Further optimization of co-culture conditions revealed that yeast extract concentration, liquid volume, and S. vaninii inoculum ratio notably influenced IPS content in the order of yeast extract concentration > liquid volume > S. vaninii inoculum ratio. Under the optimal conditions, IPS content reached 69.9626 mg/g, a 17.04% increase from pre-optimization co-culture conditions. Antioxidant capacity testing demonstrated that co-cultured IPS exhibited greater scavenging abilities for DPPH and ABTS free radicals compared to single strain cultures. These findings highlight the potential of co-culturing S. vaninii and P. sapidus to enhance IPS content and improve antioxidant capacity, presenting an effective strategy for increasing fungal polysaccharide production.
摘要：
真菌多糖通常作为天然和安全的免疫调节剂用于食品工业和生物医学领域。共培养是提高次生代谢产物产量的有价值的方法。本研究以胞内多糖(IPS)含量为筛选指标,将七种不同的真菌与香菇共培养。通过筛选选择种子预培养液培养时间,使用单因素实验评估条件，Plackett-Burman（PB）设计，和响应面方法(RSM)优化。进行了RSM优化，导致抗氧化能力的测量。结果表明，Vaninii和侧耳的共培养表现出最有效的结果。具体来说,将S.vaninii和P.sapidus种子培养物预培养2天和0天，分别,然后共同培养，与单菌株培养相比，IPS含量显着增加。共培养条件的进一步优化表明，酵母提取物浓度，液体体积,和S.vaninii接种比例显着影响IPS含量的顺序为酵母提取物浓度>液体体积>S.vaninii接种比例。在最优条件下,IPS含量达到69.9626mg/g,与优化前的共培养条件相比增加了17.04%。抗氧化能力测试表明，与单菌株培养物相比，共培养的IPS对DPPH和ABTS自由基具有更大的清除能力。这些发现突出了共同培养S.vaninii和P.sapidus以提高IPS含量和提高抗氧化能力的潜力，提出了增加真菌多糖产量的有效策略。
