PDF(Pubmed)
Abstract：
UNASSIGNED: Type 1 Diabetes Mellitus (T1DM) is one of the most common endocrine disorders of childhood and adolescence, showing a rapidly increasing prevalence worldwide. A study indicated that the composition of the oropharyngeal and gut microbiota changed in T1DM. However, no studies have yet associated the changes between the microbiomes of the oropharyngeal and intestinal sites, nor between the flora and clinical indicators. In this study, we examined the composition and characteristics of oropharyngeal and intestinal flora in patients with T1DM in compared to healthy children. We identified correlations between oropharyngeal and intestinal flora and evaluated their association with clinical laboratory tests in patients with T1DM.
UNASSIGNED: The oropharyngeal and fecal samples from 13 T1DM and 20 healthy children were analyzed by high-throughput sequencing of the V3-V4 region of 16S rRNA. The associations between microbes and microorganisms in oropharyngeal and fecal ecological niches, as well as the correlation between these and clinical indicators were further analyzed.
UNASSIGNED: It was revealed that T1DM children had distinct microbiological characteristics, and the dominant oropharyngeal microbiota genus included Streptococcus, Prevotella, Leptotrichia, and Neisseria; that of intestinal microbiota included Blautia, Fusicatenibacter, Bacteroides, and Eubacterium_hallii_group. Furthermore, oropharyngeal Staphylococcus was significantly positively correlated with intestinal norank_f__Ruminococcaceae and Ruminococcus_torques_group in TIDM children. Moreover, in these children, differential genes in oropharyngeal and intestinal samples were enriched in metabolic pathways such as amino acid generation, fatty acid metabolism, and nucleotide sugar biosynthesis. Additionally, correlation analysis between the oropharyngeal/intestinal microbiome with laboratory tests showed significant correlations between several bacterial taxa in the oropharynx and intestines and glycated hemoglobin and C-peptide.
UNASSIGNED: Unique microbial characteristics were found in the oropharynx and intestine in children with T1DM compared to healthy children. Positive correlations were found between changes in the relative abundance of oropharyngeal and gut microbiota in children with T1DM. Associations between the oropharyngeal/intestinal microbiota and laboratory investigations in children with T1DM suggest that the composition of the oropharyngeal and intestinal flora in children with T1DM may have some impact on glycemic control.
UNASSIGNED: The oropharyngeal and fecal samples from 13 T1DM and 20 healthy children were analyzed by high-throughput sequencing of the V3-V4 region of 16S rRNA. The associations between microbes and microorganisms in oropharyngeal and fecal ecological niches, as well as the correlation between these and clinical indicators were further analyzed.
UNASSIGNED: It was revealed that T1DM children had distinct microbiological characteristics, and the dominant oropharyngeal microbiota genus included Streptococcus, Prevotella, Leptotrichia, and Neisseria; that of intestinal microbiota included Blautia, Fusicatenibacter, Bacteroides, and Eubacterium_hallii_group. Furthermore, oropharyngeal Staphylococcus was significantly positively correlated with intestinal norank_f__Ruminococcaceae and Ruminococcus_torques_group in TIDM children. Moreover, in these children, differential genes in oropharyngeal and intestinal samples were enriched in metabolic pathways such as amino acid generation, fatty acid metabolism, and nucleotide sugar biosynthesis. Additionally, correlation analysis between the oropharyngeal/intestinal microbiome with laboratory tests showed significant correlations between several bacterial taxa in the oropharynx and intestines and glycated hemoglobin and C-peptide.
UNASSIGNED: Unique microbial characteristics were found in the oropharynx and intestine in children with T1DM compared to healthy children. Positive correlations were found between changes in the relative abundance of oropharyngeal and gut microbiota in children with T1DM. Associations between the oropharyngeal/intestinal microbiota and laboratory investigations in children with T1DM suggest that the composition of the oropharyngeal and intestinal flora in children with T1DM may have some impact on glycemic control.
摘要：
■1型糖尿病（T1DM）是儿童和青春期最常见的内分泌紊乱之一，在全球范围内迅速增加的患病率。一项研究表明，T1DM患者口咽和肠道菌群的组成发生了变化。然而,还没有研究将口咽部和肠道部位的微生物组之间的变化联系起来，也不在菌群和临床指标之间。在这项研究中,我们研究了与健康儿童相比，T1DM患者口咽和肠道菌群的组成和特征。我们确定了口咽和肠道菌群之间的相关性，并评估了它们与T1DM患者临床实验室检查的相关性。
■通过对16SrRNA的V3-V4区进行高通量测序，分析了13例T1DM和20例健康儿童的口咽和粪便样本。口咽和粪便生态位中微生物和微生物之间的关联，以及这些指标与临床的相关性进行进一步分析。
■提示T1DM患儿具有明显的微生物学特征，优势口咽微生物群包括链球菌，普雷沃氏菌,Leptotrichia,和奈瑟菌；肠道微生物群包括布劳特氏菌，镰刀菌,拟杆菌,和真细菌组。此外，在TIDM儿童中，口咽葡萄球菌与肠道norank_f__Ruminoccaceae和Ruminococcus_torques_组呈显着正相关。此外，在这些孩子身上，口咽和肠道样品中的差异基因在氨基酸生成等代谢途径中富集，脂肪酸代谢，和核苷酸糖生物合成。此外,口咽部/肠道微生物组与实验室检查的相关性分析显示,口咽部和肠道中的几种细菌分类群与糖化血红蛋白和C肽之间存在显著相关性.
■与健康儿童相比，在T1DM儿童的口咽部和肠道中发现了独特的微生物特征。发现T1DM儿童口咽和肠道菌群相对丰度变化呈正相关。T1DM患儿口咽/肠道菌群与实验室检查之间的关联表明，T1DM患儿口咽和肠道菌群的组成可能对血糖控制有一定影响。
■通过对16SrRNA的V3-V4区进行高通量测序，分析了13例T1DM和20例健康儿童的口咽和粪便样本。口咽和粪便生态位中微生物和微生物之间的关联，以及这些指标与临床的相关性进行进一步分析。
■提示T1DM患儿具有明显的微生物学特征，优势口咽微生物群包括链球菌，普雷沃氏菌,Leptotrichia,和奈瑟菌；肠道微生物群包括布劳特氏菌，镰刀菌,拟杆菌,和真细菌组。此外，在TIDM儿童中，口咽葡萄球菌与肠道norank_f__Ruminoccaceae和Ruminococcus_torques_组呈显着正相关。此外，在这些孩子身上，口咽和肠道样品中的差异基因在氨基酸生成等代谢途径中富集，脂肪酸代谢，和核苷酸糖生物合成。此外,口咽部/肠道微生物组与实验室检查的相关性分析显示,口咽部和肠道中的几种细菌分类群与糖化血红蛋白和C肽之间存在显著相关性.
■与健康儿童相比，在T1DM儿童的口咽部和肠道中发现了独特的微生物特征。发现T1DM儿童口咽和肠道菌群相对丰度变化呈正相关。T1DM患儿口咽/肠道菌群与实验室检查之间的关联表明，T1DM患儿口咽和肠道菌群的组成可能对血糖控制有一定影响。
