UNASSIGNED: The forty mid-lactation goats were randomly divided into two artificial environments a control group and a heat-stressed group. This study was collected from two periods, 1 day and 28 days. The first day was defined as control 1 (CT1) and HS 1 (acute HS), and the last day was defined as CT28 and HS28 (chronic HS). On the first and last day, 6 dairy goats in each group were randomly selected to collect rumen liquid after the morning feeding through oral stomach tubes. The barn temperature and humidity were recorded every day.
UNASSIGNED: Disruption of the rumen microbiome was observed under chronic HS, represented by an increase in the abundance of Prevotella and Bacteroidales (p<0.05), and upregulation of carbohydrate transport and metabolism functions (p<0.05). Additionally, the abundance of Succinimonas and Ruminobacter in chronic HS is lower than in acute HS (p<0.05), and the functions of intracellular trafficking, secretion and vesicular transport, and the cytoskeleton were downregulated (p<0.05).
UNASSIGNED: HS affected the interaction between the microbiota and host, thereby regulating milk production in dairy goats. These findings increased understanding of the crosstalk between hosts and microorganisms.
■将40只泌乳中期山羊随机分为两个人工环境,对照组和热应激组。这项研究是从两个时期收集的,1天和28天。第一天定义为对照1(CT1)和HS1(急性HS),最后一天定义为CT28和HS28(慢性HS)。在第一天和最后一天,随机抽取每组6只奶山羊,于早晨经口胃管饲喂后收集瘤胃液。每天记录谷仓温度和湿度。
■在慢性HS下观察到瘤胃微生物组的破坏,以普雷沃氏菌和拟杆菌的丰度增加(p<0.05)为代表,以及碳水化合物运输和代谢功能的上调(p<0.05)。此外,慢性HS中的丁香单胞菌和反刍动物的丰度低于急性HS(p<0.05),细胞内贩运的功能,分泌和囊泡运输,细胞骨架下调(p<0.05)。
■HS影响了微生物群与宿主之间的相互作用,从而调节奶山羊的产奶量。这些发现增加了对宿主和微生物之间串扰的理解。