Abstract：
The chromatographic behavior of the selected compounds was studied under conditions of hydrophilic interaction liquid chromatography (HILIC). The effect of mobile phase composition on the retention in different chromatographic systems was systematically examined using high-performance thin-layer chromatography. The sorbents of different polarity and adsorption characteristics were selected and mixtures of water and organic solvents of various compositions, from pure water to pure organic solvent were used as mobile phases. Increasing the amount of water in the mobile phase leads to a conversion of the separation mechanism, and the retention curves have a characteristic \"U\" shape. The conversion between the adsorption and partition mechanisms is most likely continuous and depends on the chemical nature of separated substances, the stationary phase as well as on organic component of the mobile phase. Silica gel can be considered the most suitable stationary phase for the systematic investigation of the chromatographic behavior of the test compounds, whereas acetonitrile was the most suitable solvent. The obtained results contribute to the understanding of the dominant separation mechanism, the type, and the intensity of the interactions between separated substances with both stationary and mobile phases. Besides, the lipophilicity parameters obtained under HILIC conditions were evaluated and correlated with the calculated values.
摘要：
在亲水相互作用液相色谱（HILIC）条件下研究了所选化合物的色谱行为。使用高效薄层色谱法系统地检查了流动相组成对不同色谱系统中保留率的影响。选择了不同极性和吸附特性的吸附剂，并将水和各种组成的有机溶剂混合，从纯水到纯有机溶剂用作流动相。增加流动相中的水量导致分离机制的转变，并且保留曲线具有特征“U”形。吸附和分配机制之间的转换很可能是连续的，并且取决于分离物质的化学性质，固定相以及流动相的有机组分。硅胶可以被认为是对测试化合物的色谱行为进行系统研究的最合适的固定相，而乙腈是最合适的溶剂。获得的结果有助于理解主导分离机制，类型,以及分离物质与固定相和流动相之间的相互作用强度。此外,评估了在HILIC条件下获得的亲脂性参数，并将其与计算值相关联。
