PDF(Pubmed)
Abstract：
BACKGROUND: Erythritol, a natural polyol, is a low-calorie sweetener synthesized by a number of microorganisms, such as Moniliella pollinis. Yet, a widespread use of erythritol is limited by high production costs due to the need for cultivation on glucose-rich substrates. This study explores the potential of using Trichoderma reesei as an alternative host for erythritol production, as this saprotrophic fungus can be cultivated on lignocellulosic biomass residues. The objective of this study was to evaluate whether such an alternative host would lead to a more sustainable and economically viable production of erythritol by identifying suitable carbon sources for erythritol biosynthesis, the main parameters influencing erythritol biosynthesis and evaluating the feasibility of scaling up the defined process.
RESULTS: Our investigation revealed that T. reesei can synthesize erythritol from glucose but not from other carbon sources like xylose and lactose. T. reesei is able to consume erythritol, but it does not in the presence of glucose. Among nitrogen sources, urea and yeast extract were more effective than ammonium and nitrate. A significant impact on erythritol synthesis was observed with variations in pH and temperature. Despite successful shake flask experiments, the transition to bioreactors faced challenges, indicating a need for further scale-up optimization.
CONCLUSIONS: While T. reesei shows potential for erythritol production, reaching a maximum concentration of 1 g/L over an extended period, its productivity could be improved by optimizing the parameters that affect erythritol production. In any case, this research contributes valuable insights into the polyol metabolism of T. reesei, offering potential implications for future research on glycerol or mannitol production. Moreover, it suggests a potential metabolic association between erythritol production and glycolysis over the pentose phosphate pathway.
RESULTS: Our investigation revealed that T. reesei can synthesize erythritol from glucose but not from other carbon sources like xylose and lactose. T. reesei is able to consume erythritol, but it does not in the presence of glucose. Among nitrogen sources, urea and yeast extract were more effective than ammonium and nitrate. A significant impact on erythritol synthesis was observed with variations in pH and temperature. Despite successful shake flask experiments, the transition to bioreactors faced challenges, indicating a need for further scale-up optimization.
CONCLUSIONS: While T. reesei shows potential for erythritol production, reaching a maximum concentration of 1 g/L over an extended period, its productivity could be improved by optimizing the parameters that affect erythritol production. In any case, this research contributes valuable insights into the polyol metabolism of T. reesei, offering potential implications for future research on glycerol or mannitol production. Moreover, it suggests a potential metabolic association between erythritol production and glycolysis over the pentose phosphate pathway.
摘要：
背景：赤藓糖醇，一种天然多元醇,是由许多微生物合成的低热量甜味剂，比如传粉念珠。然而，由于需要在富含葡萄糖的底物上培养,赤藓糖醇的广泛使用受到高生产成本的限制。这项研究探讨了使用里氏木霉作为赤藓糖醇生产替代宿主的潜力，因为这种腐生真菌可以在木质纤维素生物质残留物上培养。这项研究的目的是通过确定赤藓糖醇生物合成的合适碳源来评估这种替代宿主是否会导致更可持续和经济可行的赤藓糖醇生产。影响赤藓糖醇生物合成的主要参数，并评估扩大定义过程的可行性。
结果：我们的研究表明，里氏木霉可以从葡萄糖合成赤藓糖醇，但不能从木糖和乳糖等其他碳源合成赤藓糖醇。里氏酵母能够食用赤藓糖醇，但它不存在葡萄糖。在氮源中，尿素和酵母提取物比铵和硝酸盐更有效。随着pH和温度的变化，观察到对赤藓糖醇合成的显着影响。尽管成功的摇瓶实验，向生物反应器的过渡面临挑战，表明需要进一步放大优化。
结论：虽然里氏木霉显示出产赤藓糖醇的潜力，在延长的时间内达到1g/L的最大浓度，可以通过优化影响赤藓糖醇生产的参数来提高其生产率。无论如何,这项研究为里氏木霉的多元醇代谢提供了有价值的见解，为甘油或甘露醇生产的未来研究提供潜在的启示。此外，这表明赤藓糖醇的产生与戊糖磷酸途径的糖酵解之间存在潜在的代谢关联。
结果：我们的研究表明，里氏木霉可以从葡萄糖合成赤藓糖醇，但不能从木糖和乳糖等其他碳源合成赤藓糖醇。里氏酵母能够食用赤藓糖醇，但它不存在葡萄糖。在氮源中，尿素和酵母提取物比铵和硝酸盐更有效。随着pH和温度的变化，观察到对赤藓糖醇合成的显着影响。尽管成功的摇瓶实验，向生物反应器的过渡面临挑战，表明需要进一步放大优化。
结论：虽然里氏木霉显示出产赤藓糖醇的潜力，在延长的时间内达到1g/L的最大浓度，可以通过优化影响赤藓糖醇生产的参数来提高其生产率。无论如何,这项研究为里氏木霉的多元醇代谢提供了有价值的见解，为甘油或甘露醇生产的未来研究提供潜在的启示。此外，这表明赤藓糖醇的产生与戊糖磷酸途径的糖酵解之间存在潜在的代谢关联。
