PDF(Pubmed)
Abstract：
BACKGROUND: The aim of this study was to evaluate the adhesion of Candida glabrata, Candida albicans, Candida krusei, Candida parapsilosis and Candida tropicalis yeasts to disk-shaped resin materials produced from resin which used in the production of surgical guide with 0, 45 and 90-degrees printing orientations by Liquid Crystal Display additive manufacturing technology.
METHODS: Disk-shaped specimens were printed with surgical guide resin using the Liquid Crystal Display production technique in 3 printing orientations (0, 45 and 90-degrees). Surface roughness and contact angle values were evaluated. Real-Time PCR analysis was performed to evaluate Candida adhesion (C. glabrata, C. albicans, C. krusei, C. parapsilosis and C. tropicalis) Field emission scanning electron microscope (FESEM) images of the materials were obtained.
RESULTS: Specimens oriented at 45-degrees demonstrated higher surface roughness (P < .05) and lower contact angle values than other groups. No significant difference was found in the adhesion of C. glabrata, C. albicans, and C. parapsilosis among specimens printed at 0, 45, and 90-degrees orientations (P > .05). A higher proportion of C. krusei and C. tropicalis was found in the specimens printed at orientation degrees of 45 = 90 < 0 with statistical significance. Analyzing the adhesion of all Candida species reveals no statistical disparity among the printing orientations.
CONCLUSIONS: The surface roughness, contact angle, and adhesion of certain Candida species are affected by printing orientations. Hence, careful consideration of the printing orientation is crucial for fabricating products with desirable properties. In 45-degree production, roughness increases due to the layered production forming steps, whereas in 0-degree production, certain Candida species exhibit high adhesion due to the formation of porous structures. Consequently, considering these factors, it is advisable to opt for production at 90-degrees, while also considering other anticipated characteristics.
METHODS: Disk-shaped specimens were printed with surgical guide resin using the Liquid Crystal Display production technique in 3 printing orientations (0, 45 and 90-degrees). Surface roughness and contact angle values were evaluated. Real-Time PCR analysis was performed to evaluate Candida adhesion (C. glabrata, C. albicans, C. krusei, C. parapsilosis and C. tropicalis) Field emission scanning electron microscope (FESEM) images of the materials were obtained.
RESULTS: Specimens oriented at 45-degrees demonstrated higher surface roughness (P < .05) and lower contact angle values than other groups. No significant difference was found in the adhesion of C. glabrata, C. albicans, and C. parapsilosis among specimens printed at 0, 45, and 90-degrees orientations (P > .05). A higher proportion of C. krusei and C. tropicalis was found in the specimens printed at orientation degrees of 45 = 90 < 0 with statistical significance. Analyzing the adhesion of all Candida species reveals no statistical disparity among the printing orientations.
CONCLUSIONS: The surface roughness, contact angle, and adhesion of certain Candida species are affected by printing orientations. Hence, careful consideration of the printing orientation is crucial for fabricating products with desirable properties. In 45-degree production, roughness increases due to the layered production forming steps, whereas in 0-degree production, certain Candida species exhibit high adhesion due to the formation of porous structures. Consequently, considering these factors, it is advisable to opt for production at 90-degrees, while also considering other anticipated characteristics.
摘要：
背景：这项研究的目的是评估光滑念珠菌的粘附，白色念珠菌,克鲁斯念珠菌,近扁平念珠菌和热带念珠菌酵母到由树脂生产的盘状树脂材料，用于通过液晶显示器增材制造技术生产具有0、45和90度打印取向的手术导向器。
方法：使用液晶显示器生产技术以3个印刷方向（0、45和90度）用手术导向树脂印刷盘形标本。评估表面粗糙度和接触角值。进行实时PCR分析以评估念珠菌粘附(C.glabrata,C.白色念珠菌,C.Krusei,获得了材料的场发射扫描电子显微镜（FESEM）图像。
结果：与其他组相比，以45度取向的样品显示出更高的表面粗糙度（P<.05）和更低的接触角值。无明显差异的C.glabrata的粘附，C.白色念珠菌,在0度、45度和90度方向打印的标本中（P>.05）。在以45=90<0的取向度打印的样本中发现更高比例的克氏梭菌和热带梭菌,具有统计学意义。分析所有念珠菌物种的粘附性显示印刷取向之间没有统计差异。
结论：表面粗糙度，接触角,某些念珠菌的粘附受到印刷方向的影响。因此，仔细考虑印刷方向对于制造具有所需性能的产品至关重要。在45度生产中，由于分层生产成型步骤，粗糙度增加，而在0度生产中，某些念珠菌物种由于多孔结构的形成而表现出高粘附性。因此,考虑到这些因素，建议选择90度生产，同时还要考虑其他预期特征。
方法：使用液晶显示器生产技术以3个印刷方向（0、45和90度）用手术导向树脂印刷盘形标本。评估表面粗糙度和接触角值。进行实时PCR分析以评估念珠菌粘附(C.glabrata,C.白色念珠菌,C.Krusei,获得了材料的场发射扫描电子显微镜（FESEM）图像。
结果：与其他组相比，以45度取向的样品显示出更高的表面粗糙度（P<.05）和更低的接触角值。无明显差异的C.glabrata的粘附，C.白色念珠菌,在0度、45度和90度方向打印的标本中（P>.05）。在以45=90<0的取向度打印的样本中发现更高比例的克氏梭菌和热带梭菌,具有统计学意义。分析所有念珠菌物种的粘附性显示印刷取向之间没有统计差异。
结论：表面粗糙度，接触角,某些念珠菌的粘附受到印刷方向的影响。因此，仔细考虑印刷方向对于制造具有所需性能的产品至关重要。在45度生产中，由于分层生产成型步骤，粗糙度增加，而在0度生产中，某些念珠菌物种由于多孔结构的形成而表现出高粘附性。因此,考虑到这些因素，建议选择90度生产，同时还要考虑其他预期特征。
