PDF(Pubmed)
Abstract：
Circulating proteins may provide insights into the varying biological mechanisms involved in heart failure (HF) with preserved ejection fraction (HFpEF) and reduced ejection fraction (HFrEF). We aimed to identify specific proteomic patterns for HF, by comparing proteomic profiles across the ejection fraction spectrum. We investigated 4210 circulating proteins in 739 patients with normal (Stage A/Healthy) or elevated (Stage B) filling pressures, HFpEF, or ischemic HFrEF (iHFrEF). We found 2122 differentially expressed proteins between iHFrEF-Stage A/Healthy, 1462 between iHFrEF-HFpEF and 52 between HFpEF-Stage A/Healthy. Of these 52 proteins, 50 were also found in iHFrEF vs. Stage A/Healthy, leaving SLITRK6 and NELL2 expressed in lower levels only in HFpEF. Moreover, 108 proteins, linked to regulation of cell fate commitment, differed only between iHFrEF-HFpEF. Proteomics across the HF spectrum reveals overlap in differentially expressed proteins compared to stage A/Healthy. Multiple proteins are unique for distinguishing iHFrEF from HFpEF, supporting the capacity of proteomics to discern between these conditions.
摘要：
循环蛋白可以提供对心力衰竭（HF）与保留的射血分数（HFpEF）和降低的射血分数（HFrEF）有关的各种生物学机制的见解。我们旨在确定HF的特定蛋白质组学模式，通过比较整个射血分数光谱的蛋白质组学图谱。我们调查了739例正常（A期/健康）或升高（B期）充盈压患者的4210种循环蛋白，HFpEF,或缺血性HFrEF(iHFrEF)。我们发现iHFrEFA期/健康之间有2122种差异表达的蛋白质，iHFrEF-HFpEF之间的1462和HFpEF-A期/健康之间的52。在这52种蛋白质中，在iHFrEF与阶段A/健康，留下SLITRK6和NELL2仅在HFpEF中以较低水平表达。此外，108种蛋白质,与细胞命运承诺的调节有关，仅在iHFrEF-HFpEF之间有所不同。与阶段A/健康相比，整个HF谱的蛋白质组学揭示了差异表达蛋白质的重叠。多种蛋白质在区分iHFrEF和HFpEF方面是独特的，支持蛋白质组学辨别这些条件的能力。
