PDF(Pubmed)
Abstract：
BACKGROUND: 3D-printing is widely used in regenerative medicine and is expected to achieve vaginal morphological restoration and true functional reconstruction. Mesenchymal stem cells-derived exosomes (MSCs-Exos) were applyed in the regeneration of various tissues. The current study aimed to explore the effctive of MSCs-Exos in vaginal reconstruction.
METHODS: In this work, hydrogel was designed using decellularized extracellular matrix (dECM) and gelatin methacrylate (GelMA) and silk fibroin (SF). The biological scaffolds were constructed using desktop-stereolithography. The physicochemical properties of the hydrogels were evaluated; Some experiments have been conducted to evaluate exosomes\' effect of promotion vaginal reconstruction and to explore the mechanism in this process.
RESULTS: It was observed that the sustained release property of exosomes in the hydrogel both in vitro and in vitro.The results revealed that 3D scaffold encapsulating exosomes expressed significant effects on the vascularization and musule regeneration of the regenerative vagina tissue. Also, MSCs-Exos strongly promoted vascularization in the vaginal reconstruction of rats, which may through the PI3K/AKT signaling pathway.
CONCLUSIONS: The use of exosome-hydrogel composites improved the epithelial regeneration of vaginal tissue, increased angiogenesis, and promoted smooth muscle tissue regeneration. 3D-printed, lumenal scaffold encapsulating exosomes might be used as a cell-free alternative treatment strategy for vaginal reconstruction.
METHODS: In this work, hydrogel was designed using decellularized extracellular matrix (dECM) and gelatin methacrylate (GelMA) and silk fibroin (SF). The biological scaffolds were constructed using desktop-stereolithography. The physicochemical properties of the hydrogels were evaluated; Some experiments have been conducted to evaluate exosomes\' effect of promotion vaginal reconstruction and to explore the mechanism in this process.
RESULTS: It was observed that the sustained release property of exosomes in the hydrogel both in vitro and in vitro.The results revealed that 3D scaffold encapsulating exosomes expressed significant effects on the vascularization and musule regeneration of the regenerative vagina tissue. Also, MSCs-Exos strongly promoted vascularization in the vaginal reconstruction of rats, which may through the PI3K/AKT signaling pathway.
CONCLUSIONS: The use of exosome-hydrogel composites improved the epithelial regeneration of vaginal tissue, increased angiogenesis, and promoted smooth muscle tissue regeneration. 3D-printed, lumenal scaffold encapsulating exosomes might be used as a cell-free alternative treatment strategy for vaginal reconstruction.
摘要：
背景：3D打印广泛用于再生医学，有望实现阴道形态恢复和真正的功能重建。间充质干细胞来源的外泌体（MSC-Exos）应用于各种组织的再生。本研究旨在探讨MSCs-Exos在阴道重建中的作用。
方法：在这项工作中，使用脱细胞细胞外基质(dECM)和明胶甲基丙烯酸酯(GelMA)和丝素蛋白(SF)设计水凝胶。使用桌面立体光刻法构建生物支架。评价了水凝胶的理化性质；进行了一些实验来评估外泌体促进阴道重建的作用，并探讨了这一过程的机制。
结果:观察到外泌体在水凝胶中在体外和体外的持续释放性质。结果表明，3D支架包裹外泌体对再生阴道组织的血管化和胶囊再生有显著影响。此外，MSCs-Exos强烈促进大鼠阴道重建血管化,可能通过PI3K/AKT信号通路。
结论：外泌体-水凝胶复合材料的使用改善了阴道组织的上皮再生，血管生成增加，促进平滑肌组织再生。3D打印,封装外泌体的腔内支架可用作阴道重建的无细胞替代治疗策略.
方法：在这项工作中，使用脱细胞细胞外基质(dECM)和明胶甲基丙烯酸酯(GelMA)和丝素蛋白(SF)设计水凝胶。使用桌面立体光刻法构建生物支架。评价了水凝胶的理化性质；进行了一些实验来评估外泌体促进阴道重建的作用，并探讨了这一过程的机制。
结果:观察到外泌体在水凝胶中在体外和体外的持续释放性质。结果表明，3D支架包裹外泌体对再生阴道组织的血管化和胶囊再生有显著影响。此外，MSCs-Exos强烈促进大鼠阴道重建血管化,可能通过PI3K/AKT信号通路。
结论：外泌体-水凝胶复合材料的使用改善了阴道组织的上皮再生，血管生成增加，促进平滑肌组织再生。3D打印,封装外泌体的腔内支架可用作阴道重建的无细胞替代治疗策略.
