Abstract：
Establishing humane endpoints to minimize animal suffering in studies on snake venom toxicity and antivenom potency tests is crucial. Our findings reveal that Swiss mice exhibit early temperature drop following exposure to different snake venoms and combinations of venoms and antivenoms, predicting later mortality. Evaluating temperature we can identify within 3 h post-inoculation, the animals that will not survive in a period of 48 h. Implementing temperature as a criterion would significantly reduce animal suffering in these studies without compromising the outcomes.
摘要：
在蛇毒毒性和抗血清效力测试的研究中，建立人道的终点以最大程度地减少动物的痛苦至关重要。我们的发现表明，瑞士小鼠在暴露于不同的蛇毒以及毒液和抗蛇毒血清的组合后表现出早期温度下降，预测以后的死亡率。评估温度，我们可以在接种后3小时内确定，在48小时内无法存活的动物。在这些研究中，将温度作为标准将大大减少动物的痛苦，而不会影响结果。
